[Transglutaminase 3] Human epidermal transglutaminase [T024]_ZEDIRA - 코아사이언스

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Human epidermal transglutaminase

(TG3, recombinantly produced in insect cells)

Cat.# T024 / Size: 200 ug

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Documents

PDS MSDS

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Synonym

TG3, TGase 3, epidermal protein-glutamine-γ-glutamyltransferase, TGE

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Background info

This product replaces product replaces product number T124.

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Molecular Weight

78 kDa

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Source

Recombinantly produced in insect cells

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Activation

His6-rhTG3 is a Ca2+-dependent enzyme.

10 μl His6-rhTG3 (1 μg/μl)

35 μl 50 mM Tris-HCl pH 8

5 μl Dispase I (Roche) (0.2 μg/μl)

Incubate for 20 min at 30°C

Add 10 mM Ca2+ when measuring His6-rhTG3 activity.

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Specific Activity

> 1000 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG3-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231)].

1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml).

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Appearance

White lyophilized solid.

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Description

His6-rhTG3 is based on the TGM3-allele from clone DKFZp686J0716 (isolated from different tissues), corrected by the insertion of the missing T at Position 435. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAALGV… . His6-rhTG3 is produced in insect cells and purified by ion metal chelating and ion exchange chromatography to more than 95 % purity.

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Reagents

The Transglutaminase is lyophilized from 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA and 1 mM DTT. Sample contains maltodextrin.

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Reconstitution

Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.

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Application

His6-rhTG3 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG3 may also be used for immunoprecipitation and the detection of TG3 autoantibodies.

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Storage

Storage for several months is possible at ≤ - 20°C.

Delivery is possible at ambient temperature

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Reference(s)

Hietikko et al., Acta. Derm. Venereol. 2018, 98:366-72

Yamane et al., FEBS J. 2010, 277:3564-74

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Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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This product replaces TG3, recombinantly produced in E. coli (T012).

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Related Products

T057: Inhibited human epidermal transglutaminase

T013: Human epidermal transglutaminase, Dispase activated

T101: 1,3,4,5-Tetramethyl-2-[(2-oxopropyl)thio]imidazolium chloride

T036: Transglutaminase Assay Kit ("DCC"), fluorescent

A015: Polyclonal antibody to human epidermal transglutaminase (TG3)

A030: FITC-labeled polyclonal antibody to human TG3

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