[Transglutaminase fluorogenic Activity Assay] Transglutaminase Assay Kit ("DCC"), fluorescent [T036]_ZEDIRA - 코아사이언스

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Transglutaminase Assay Kit ("DCC"), fluorescent

Casein, Dansylcadaverine

Cat.# T036 / Size: 1 kit

Documents

 PDS  MSDS

Description

Assay principle

Transglutaminases catalyze acyl transfer reactions from glutamine residues in proteins or peptides to primary amines. The transglutaminase-catayzed covalent coupling of monodansylcadaverine into N,N-dimethylcasein produces a shift in intensity and wavelength of fluorescence of the dansyl group. The transglutaminase activity can be monitored online by measurement of the fluorescence (excitation wavelength 332 nm; emission wavelength 500 nm). The relative transglutaminase activity is shown by increase of fluorescence intensity over time.

Reagents in the kit

(1) SUBSTRATE REAGENT: 2 x 0,185 mg monodansylcadaverine
(2) BUFFER REAGENT: 2 x 20 ml lyophilized TRIS buffer pH 8,0 containing calcium chloride, sodium chloride, N,N-dimethylcasein, glutathione.

Equipment

The Fluorescent Transglutaminase Assay can be used in standard fluorescence spectrophotometers. Refer to the instructions of the manufacturer.

Intended use

Determination of transglutaminase activity (hTG1, hTG2, hTG6).

Reference(s)

Lorand et al., Anal. Biochem. 1971, 44:221-31

Note

Intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

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