Transglutaminase 1 [T009]_ZEDIRA - 코아사이언스

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Transglutaminase 1

Cat.# T009 / Size: 150 ug

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Documents

PDS MSDS

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Synonym

TG1, TGase 1, keratinocyte protein-glutamine-γ-glutamyltransferase

Molecular Weight

90 kDa

Source

Recombinant, produced in E. coli

Purity

> 90 % (visually by SDS-PAGE)

Activation

Add 10 mM Ca2+ to activate His6-rhTG1.

Activity

> 2,500 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG1-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231)].

1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].

Appearance

White lyophilized solid.

Description

His6-rhTG1 is based on the TGM1-allele from I.M.A.G.E.-clone IRAKp961M1628 isolated from human skin squamous cell carcinoma. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHMDGPR. His6-rhTG1 is purified by IMAC to more than 90 % purity.

Reagents

The Transglutaminase is lyophilized from 50 mM Tris-HCl pH 8.0, 10 mM Glutathion

Reconstitution

Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage.

Application

His6-rhTG1 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG1 may also be used for immunoprecipitation.

Storage

Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.

Delivery at ambient temperature is possible

Reference(s)

Chrobok et al., PLoS One. 2018, 13:e0196433

Plank et al., J Invest Dermatol. 2018, pii: S0022-202X(18)32817-3

Fischer et al., J. Invest. Dermatol. 2013, 133:1170-7

Fukui et al., FEBS J. 2013, 280:1420-9

Schaertl et al., J. Biomol. Screen. 2010, 15:478-87

Yamane et al., FEBS J. 2010, 277:3564-74

Note

Intended for research use only, not for use in human, therapeutic or diagnostic applications.

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Related Products

T035: Human keratinocyte transglutaminase

A018: Polyclonal antibody to human keratinocyte transglutaminase (TG1)

A029: FITC-labeled polyclonal antibody to human keratinocyte transglutaminase (TG1)

T101: 1,3,4,5-Tetramethyl-2-[(2-oxopropyl)thio]imidazolium chloride

T036: Transglutaminase Assay Kit ("DCC"), fluorescent

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Note: INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

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