[Veterinary & Agriculture][qPCR 키트] Dichelobacter nodosus & Fusobacter necrophorum qPCR Kit [MD06221]_NZYTech - 코아사이언스

 

Dichelobacter nodosus & Fusobacter necrophorum qPCR Kit

Cat.# MD06221 / Size: 100 reactions

Manual / MSDS

Dichelobacter nodosus & Fusobacter necrophorum qPCR Kit is designed for the in vitro detection of D. nodosus and F. necrophorum genomes.

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Dichelobacter nodosus and Fusobacter necrophorum are two Gram-negative, nonmotile and anaerobic bacteria. These two bacteria can work synergistically causing footrot (infectious pododermatitis), a hoof infection commonly found in sheep, goats and cattle. It is responsible for the majority of cases of lameness in sheep. Dichelobacter nodosus is the main cause of the disease, while Fusobacterium necrophorum is an associated pathogen.

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Dichelobacter nodosus & Fusobacter necrophorum qPCR Kit is designed for the in vitro detection of D. nodosus and F. necrophorum subsp. necrophorum genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to these species. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of D. nodosus and F. necrophorum genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Dichelobacter nodosus & Fusobacter necrophorum
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Triplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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