[Veterinary & Agriculture][소 토로바이러스 검출 RT-qPCR 키트] Bovine torovirus RT-qPCR Kit [MD06041]_NZYTech - 코아사이언스

 

Bovine torovirus RT-qPCR Kit

Cat.# MD06041 / Size: 100 reactions

Manual / MSDS

Bovine torovirus RT-qPCR Kit is designed for the in vitro detection of BToV genomes.

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Bovine torovirus (BToV) is a positive single-stranded RNA virus that belongs to the Torovirus genus within the Tobaniviridae family. BToV is an endemic virus with incidence in cattle herds. BToV is a pathogenic virus that causes mild to profuse diarrhea in young calves. The first clinical symptoms of a BToV infection are mild fever, depression, weakness, and anorexia, followed by a greenish yellow to bright yellow watery diarrhea. Calves may develop severe dehydration and die.

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Bovine torovirus RT-qPCR Kit is designed for the in vitro detection of BToV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to BToV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of BToV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme One-step RT-qPCR Master Mix (2x)

- RT-qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- RNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Bovine torovirus
Sample Material	RNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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