[Veterinary & Agriculture][브라키스피라 검출 qPCR 키트] Brachyspira hyodysenteriae & Brachyspira pilosicoli qPCR Kit [MD06051]_NZYTech - 코아사이언스

 

Brachyspira hyodysenteriae & Brachyspira pilosicoli qPCR Kit

Cat.# MD06051 / Size: 100 reactions

Manual / MSDS

Brachyspira hyodysenteriae & Brachyspira pilosicoli qPCR Kit is designed for the in vitro detection of B. hyodysenteriae and B. pilosicoli genomes.

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Brachyspira hyodysenteriae and Brachyspira pilosicoli are Gram-negative anaerobic bacteria and are the causative agents of swine dysentery (SD) and porcine intestinal spirochetosis (PIS), respectively. While SD is characterized by mucohemorrhagic diarrhea and inflammation in the large intestine, PIS is characterized by mild colitis, diarrhea, reduced pigs growth rate and, consequently, increased time to market weight.

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Brachyspira hyodysenteriae & Brachyspira pilosicoli qPCR Kit is designed for the in vitro detection of B. hyodysenteriae and B. pilosicoli genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to B. hyodysenteriae and B. pilosicoli. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of B. hyodysenteriae and B. pilosicoli genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Brachyspira hyodysenteriae & Brachyspira pilosicoli
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

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