[Veterinary & Agriculture][히스토필루스 솜니 검출 qPCR 키트] Histophilus somni qPCR Kit [MD06251]_NZYTech - 코아사이언스

 

Histophilus somni qPCR Kit

Cat.# MD06251 / Size: 100 reactions

Manual / MSDS

Histophilus somni qPCR Kit is designed for the in vitro detection of H. somni genomes.

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Histophilus somni is a Gram-negative and facultative anaerobic bacterium belonging to the family Pasteurellaceae. This bacterium is a commensal of mucous membranes of the upper respiratory tract and reproductive tract in cattle and other small ruminants. H. somni is one of the bacterial agents responsible for Bovine Respiratory Disease (BRD) complex, which typically involves multiple pathogens residing together in biofilm environments. Other important bacterial pathogens of BRD are Pasteurella multocida, Mannheimia haemolytica, Trueperella pyogenes and Mycoplasma bovis.

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Histophilus somni qPCR Kit is designed for the in vitro detection of H. somni genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to this species. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of H. somni species. The primers and probe sequences have very high (>95%) homology with a broad range of H. somni genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Histophilus somni
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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