[Veterinary & Agriculture][만헤이미아 헤모라이티카 검출 qPCR 키트] Mannheimia haemolytica qPCR Kit [MD06301]_NZYTech - 코아사이언스

 

Mannheimia haemolytica qPCR Kit

Cat.# MD06301 / Size: 100 reactions

Manual / MSDS

 

Mannheimia haemolytica qPCR Kit is designed for the in vitro detection of M. haemolytica genomes.

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Mannheimia haemolytica is a Gram-negative bacterium that belong to the Pasteurellaceae family. This bacterium is present in the natural flora of the upper respiratory system of ruminant. It is considered an opportunistic pathogen and the main factor of pneumonic pasteurellosis, which has caused a severe economic loss in sheep and cattle industries. M. haemolytica is the causative agent of pneumonia, septicemia and gangrenous mastitis in sheep, goats and ewes, respectively.

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Mannheimia haemolytica qPCR Kit is designed for the in vitro detection of M. haemolytica genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to M. haemolytica. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of M. haemolytica genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Mannheimia haemolytica
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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