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5-Iodoacetamidofluorescein (5-IAAF)

Cat.# TF-0113-D025.0-001 / Size: 25mg

Thiol-reactive fluorescein derivative for fluorescence derivatization, especially for labeling of cysteines and thiol-modified oligos.

Properties

CAS-Number:
63368-54-7
Molecular Weight:
538,27
Empirical Formula:
C24H13NO6I
Absorption [nm]:
492 ± 3
Emission [nm]:
513 ± 3
ε [M-1 cm-1]:
>70 000
Buffer System for Fluorescence Readings:
T&S Buffer, pH 9
Solubility:
DMF
Appearance (colour):
yellow
Appearance (form):
solid (powder)
Storage:
- 20 °C. Keep dry! Keep away from light, especially in solution!
Name:
2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-(2-iodo-acetylamino)-benzoic acid
Trade name:
5-Iodoacetamidofluorescein, 5-IAAF
Reactive Moiety:
Iodoacetamide, reacts with thiol to form a stable thioether linkage

Description

Readily reacts with the thiol group found on cysteine or with thiol-modified oligonucleotides to form a stable carbon-sulfur bond.

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