[Dyes & Labeling/Fluorescent Dyes/Fluorescein Derivatives/Amine-Reactive] Fluorescein-5/6-isothiocyanate (5/6-FITC) [AF-0115][CAS no. 27072-45-3]_emp Biotech - 코아사이언스

 

Fluorescein-5/6-isothiocyanate (5/6-FITC)

Cat.# AF-0115-C050.0-001 / Size: 50ug

Cat.# AF-0115-C050.0-003 / Size: 3 x 50ug

Cat.# AF-0115-D005.0-001 / Size: 5mg

Cat.# AF-0115-D020.0-001 / Size: 20mg

Cat.# AF-0115-D100.0-001 / Size: 100mg

Cat.# AF-0115-E001.0-001 / Size: 1g

Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 494 nm, Em: 518 nm).

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Properties

CAS-Number:	27072-45-3
Molecular Weight:	389.38
Empirical Formula:	C21H11NO5S
Absorption [nm]:	494 ± 3
Emission [nm]:	518 ± 3
ε [M-1 cm-1]:	>70 000
Buffer System for Fluorescence Readings:	T&S Buffer, pH 9
Solubility:	DMF
Appearance (colour):	orange
Appearance (form):	solid (powder)
Storage:	- 20 °C. Keep dry! Keep away from light, especially in solution!
Trade name:	5/6 FITC, mixed isomers
Reactive Moiety:	Isothiocyanate, reacts with 1° amines to form a stable thiourea linkage

Description

This classic amine-reactive fluorescein derivatization reagent is widely used for covalent labeling of proteins and alkylamino modified nucleic acids.

Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.

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5/6-FITC

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