[Dyes & Labeling/Fluorescent Dyes/Fluorescein Derivatives/Amine-Reactive] 6-Carboxyfluorescein NHS ester, single isomer (6-FAM-SE) [AF-0106][CAS no. 92557-81-8]_emp Biotech - 코아사이언스

 

6-Carboxyfluorescein NHS ester, single isomer (6-FAM-SE)

Cat.# AF-0106-D020.0-001 / Size: 20mg

Cat.# AF-0106-D100.0-001 / Size: 100mg

Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 497 nm, Em: 519 nm).

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Properties

CAS-Number:	92557-81-8
Molecular Weight:	473,9
Empirical Formula:	C25H15NO9
Absorption [nm]:	492 ± 3
Emission [nm]:	514 ± 3
ε [M-1 cm-1]:	>81 000
Excitation [nm]:	498
Buffer System for Fluorescence Readings:	PBS, pH 7.4
Solubility:	Acetonitril, DMF, DMSO
Appearance (colour):	orange
Appearance (form):	solid (powder)
Storage:	- 20 °C. Keep dry! Keep away from light, especially in solution!
Name:	2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-terephthalic acid 4-(2,5-dioxo-pyrrolidin-1-yl) ester
Trade name:	6-Carboxyfluorescein-NHS-ester; 6-FAM-SE; 6-carboxyfluorescein succinimidyl ester, single isomers
Reactive Moiety:	NHS ester, reacts with 1° amines to form a stable amide linkage

Description

This single isomer amine-reactive derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.

Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications.

It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.

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