[Dyes & Labeling/Fluorescent Dyes/Fluorescein Derivatives/Non-Activated] 5-Carboxyfluorescein, single isomer (5-FAM) (99+%) [F-0105][CAS no. 76823-03-5]_emp Biotech - 코아사이언스

 

5-Carboxyfluorescein, single isomer (5-FAM) (99+%, high purity)

Cat.# F-0105-D025.0-001 / Size: 25mg

Cat.# F-0105-D100.0-001 / Size: 100mg

Cat.# F-0105-E001.0-001 / Size: 1g

Cat.# F-0105-E005.0-001 / Size: 5g

High purity, non-activated fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 493 nm, Em: 517 nm).

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Properties

CAS-Number:	76823-03-5
Molecular Weight:	376,32
Empirical Formula:	C21H12O7
Absorption [nm]:	493 ± 3
Emission [nm]:	517 ± 3
ε [M-1 cm-1]:	>72 000
Excitation [nm]:	488
Buffer System for Fluorescence Readings:	PBS, pH 7.4
Solubility:	Methanol, DMF, DMSO
Appearance (colour):	yellow
Appearance (form):	solid (powder)
Storage:	RT! Keep dry! Keep away from light, especially in solution!
Trade name:	5-Carboxyfluorescein; 5-FAM; 5-Carboxyfluorescein, single isomer
Reactive Moiety:	free carboxylic acid

Description

This single isomer non-activated fluorescein derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.

Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.

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5-FAM

Absorption: 493 nm (e = 72.000 M-1cm-1) at pH >8

Emission: 517 nm at pH >8

 

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