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[Dyes & Labeling/Fluorescent Dyes/Fluorescein Derivatives/Non-Activated] 5-Carboxyfluorescein, single isomer (5-FAM) (99+%) [F-0105][CAS no. 76823-03-5]_emp Biotech - 코아사이언스
코피디 2024. 9. 6. 16:255-Carboxyfluorescein, single isomer (5-FAM) (99+%, high purity)
Cat.# F-0105-D025.0-001 / Size: 25mg
Cat.# F-0105-D100.0-001 / Size: 100mg
Cat.# F-0105-E001.0-001 / Size: 1g
Cat.# F-0105-E005.0-001 / Size: 5g
High purity, non-activated fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 493 nm, Em: 517 nm).
Properties
CAS-Number:
|
76823-03-5
|
Molecular Weight:
|
376,32
|
Empirical Formula:
|
C21H12O7
|
Absorption [nm]:
|
493 ± 3
|
Emission [nm]:
|
517 ± 3
|
ε [M-1 cm-1]:
|
>72 000
|
Excitation [nm]:
|
488
|
Buffer System for Fluorescence Readings:
|
PBS, pH 7.4
|
Solubility:
|
Methanol, DMF, DMSO
|
Appearance (colour):
|
yellow
|
Appearance (form):
|
solid (powder)
|
Storage:
|
RT! Keep dry! Keep away from light, especially in solution!
|
Trade name:
|
5-Carboxyfluorescein; 5-FAM; 5-Carboxyfluorescein, single isomer
|
Reactive Moiety:
|
free carboxylic acid
|
Description
This single isomer non-activated fluorescein derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.
Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.
5-FAM
Absorption: 493 nm (e = 72.000 M-1cm-1) at pH >8
Emission: 517 nm at pH >8
* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.
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