[Dyes & Labeling/Fluorescent Dyes/Fluorescein Derivatives/Amine-Reactive] 5-Carboxyfluorescein NHS ester, single isomer (5-FAM-SE) [AF-0105][CAS no. -]_emp Biotech - 코아사이언스

 

5-Carboxyfluorescein NHS ester, single isomer (5-FAM-SE)

Cat.# AF-0105-D020.0-001 / Size: 20mg

Cat.# AF-0105-D100.0-001 / Size: 100mg

Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 495 nm, Em: 519 nm).

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Properties

Excitation [nm]:	496
Name:	4-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-isophthalic acid 3-(2,5-dioxo-pyrrolidin-1-yl) ester

Description

This single isomer amine-reactive derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.

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Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.

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