[Dyes & Labeling/Fluorescent Dyes/Fluorescein Derivatives/Amine-Reactive] 5/6-Carboxyfluorescein NHS ester, mixed isomers (5/6-FAM-SE) [AF-0101][CAS no. 117548-22-8]_emp Biotech - 코아사이언스

 

5/6-Carboxyfluorescein NHS ester, mixed isomers (5/6-FAM-SE)

Cat.# AF-0101-C050.0-001 / Size: 50ug

Cat.# AF-0101-C050.0-003 / Size: 3 x 50ug

Cat.# AF-0101-D020.0-001 / Size: 20mg

Cat.# AF-0101-D100.0-001 / Size: 100mg

Cat.# AF-0101-E001.0-001 / Size: 1g

Amine-reactive fluorescein dye marker for labeling of biomolecules (Abs: 496 nm, Em: 517 nm).

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Properties

CAS-Number:	117548-22-8
Molecular Weight:	473,39
Empirical Formula:	C25H15NO9
Absorption [nm]:	496 ± 3
Emission [nm]:	517 ± 3
ε [M-1 cm-1]:	>70 000
Excitation [nm]:	493 ± 3
Excitation source:	488 nm (Ar-Ion-Laser)
Buffer System for Fluorescence Readings:	T&S Buffer, pH 9
Solubility:	Acetonitrile, Acetone
Appearance (colour):	yellow orange
Appearance (form):	solid (powder)
Storage:	- 20 °C. Keep dry! Keep away from light, especially in solution!
Trade name:	5/6-Carboxyfluorescein-NHS-ester; 5/6-FAM-SE; 5-(and 6)-carboxyfluorescein succinimidyl ester, mixed isomers
Reactive Moiety:	NHS ester, reacts with 1° amines to form a stable amide linkage
Application:	labeling of antibodies, detection of proteins in cast gels and western blots (source: ThermoFisher Scientific - rephrase!)

Description

This amine-reactive fluorescein derivatization reagent is widely used for covalent labeling of proteins and alkylamino modified nucleic acids.

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Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

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Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.

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