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SpheroRuler - Calibration beads for SMLM microscopy

Calibration beads for super-resolution microscopy

1 vial of 50 µL of a SpheroRuler suspension in PBS pH 7.4

Cat.#                                     Bead type(Size/Color)      Pack Size

AFA-NAN-100-647              100nm / Far-red                  50 µL

AFA-NAN-1000-647            1um / Far-red                      50 µL

AFA-NAN-1000-488            1um / Green                        50 µL

AFA-NAN-1000-647+488   1um / Far-red + Green        50 µL

AFA-NAN-6000-647           6um / Far-red                      50 µL

SpheroRuler is a monodispersed suspension of 1µm diameter spheres coated with far-red and/or green fluorophores giving a stable blinking in SMLM microscopy. Their consistent size and geometry make them very practical and reliable standards to assess the accuracy of your x-y or z measurements, 3D reconstruction methods or your image quality. Monodispersed and immobile in solution, they can also be used as rulers, for drift correction or as guides to help localize features on your biological samples.

Kit description: 1 vial of 50 µL of a SpheroRuler suspension in PBS pH 7.4.

Allows 10 experiments when using the recommended 5 µL volume in 35 mm glass-bottom dishes.

Concentration: 3.5 x 10e6 particles per mL (6 µm beads), 7 x 10e8 particles per mL (1 µm beads) or 7 x 10e11 particles per mL (100 nm beads).

Stability: at least 7 months when stored at 4°C.

Dyes: Far-red fluorescent beads are coated with 647-fluorophores. Green fluorescent beads are coated with your choice of 488 or 495-fluorophores.

Compatible with: dSTORM, SRRF, Airyscan confocal, confocal, SEM

The SpheroRuler family is expanding !

Following the success of SpheroRuler beads, we are thrilled to introduce some brand-new SpheroRulers of various sizes and colours that are now ready to join our catalog:

Additional resources:

Visit our glossary for a definition of dSTORM and super-resolution microscopy .

*본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences Idylle France 프랑스 korea 한국 대리점 연구용 소모품 시약 세포생물학 cell imaging 세포 관찰 zebrafish imaging 제브라피쉬 이미징 관찰 UbiClear clearing kit for biological tissues SensiDeath - Ultra-sensitive human cell death assay a-Clipse - Chambers for luminescence imaging and photo/spectro-electrochemistry CrystalChip - Microfluidic chips for protein crystallization AgarSqueezer - agar-based cell compression device AgarSqueezer device AgarSqueezer - agar-based cell compression device SpheroTribe - all-in-one kit for reproducible spheroid/organoids Chitozen - Adhesive coverslips for bacteria imaging FakirSlide - Nanostructured glass coverslips for cell culture & isolated membranes Everspark - A long-lasting buffer for dSTORM​ GlowMito - Red fluorescent mitochondrial tracker BrightER - Endoplasmic reticulum probe for live imaging & flow cytometry ColorFlux - A visual indicator of bacterial efflux Stampwell V - 3D cell imaging Stampwell Z - Zebrafish imaging Stencell - Removable PDMS cell culture chambers AgarSqueezer Silicium Wafer SpheroRuler - Calibration beads for SMLM microscopy DNAbsolute - A column-free DNA extraction buffer Actiflash - A photoinducible protein activator Phimask - A phase mask to turn a 2D SMLM setup into a 3D image Stampwell U - 3D cell aggregation Luminicell Tracker™ - Cell Labelling Kit Luminicell Tracker™ - Vascular Labelling Kit