Phimask - A phase mask to turn a 2D SMLM setup into a 3D image [PBO-SEL]_Idylle - 코아사이언스

 

Phimask - A phase mask to turn a 2D SMLM setup into a 3D image

Phimask is a diffraction grating system made for tracking native cellular processes in intact tissues with the following features:

- Compatible with up to 3 colors in the 500-750nm range

- Generating interferences with <20% photon loss

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Cat.# PBO-SEL

Phimask is a super resolution imaging tool for turning a 2D SMLM setup into a 3D image.

This phase mask is meant for bioimaging analysis to track native cellular processes in intact tissues using phase encoded-positioning. It can be adapted to any microscope.

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Adding Phimask on the experiment

The PhiMask has to be placed a in front of the sensor of the camera you want to use. If the sensor is mechanically inaccessible, you can use an imaging relay.

The Phimask produces interferences (fringes) which are sampled by your camera. The fringes carry the particule localization in 3D.

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Retrieving the 3D localization

By using fluorescent smaller than the resolution of the microscope (e.g. 100 nm beads for a NA=1.4 microscope objective) and a z-axis motorized microscope, it is possible to perform a calibration of the SELFI-PSF along the z axis. This calibration can be used as a look-up-table to determine the axial z localization of each emitter during an experiment.

The lateral localization can be obtained by applying a low-pass filter to the image to remove the fringe modulation. This leads to the signal envelope which is exactly the same as recording the image with the PhiMask; regular state-of-the-art 2D localization algorithms can be applied (ex. 2D Gaussian fitting, Wavelets...).

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Visit our glossary for a definition of super-resolution microscopy.

More information on the Datasheet.

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Results

 

 

Protocol

How to use Phimask with your microscope:

• Read the full protocol of use: Phimask protocol

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Publications

Phimask has been developed by Pierre Bon and his team.

Original publication:

Bon, P., Linarès-Loyez, J., Feyeux, M., Alessandri, K., Lounis, B., Nassoy, P., & Cognet, L. (2018). Self-interference 3D super-resolution microscopy for deep tissue investigations. Nature methods, 15(6), 449–454. https://doi.org/10.1038/s41592-018-0005-3

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*본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

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