​Everspark - A long-lasting buffer for dSTORM [KMO-ETE-450-10, KMO-ETE+-450-10]_Idylle - 코아사이언스

 

Everspark - A long-lasting buffer for dSTORM

Everspark buffer at 100 mM MEA in Tris buffer

10 vials containing 450 μL each (total volume 4,5 mL)

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Cat.#                         Version                  Size

KMO-ETE-450-10    Everspark 1.0        4.5mL (10 x 450 µL)

KMO-ETE+-450-10  Everspark 1.0        4.5mL (10 x 450 µL)

 

Discover the long-lasting Everspark blinking buffer series for prolonged blinking in dSTORM microscopy. No more rushing your imaging. Mount your sample, and get stable blinking for weeks. ​​

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NEW ! Discover the newly-developed Everspark 2.0 also compatible with green fluorophores (i.e. AF488) for improved resolution in 3-colour dSTORM microscopy.

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Everspark 1.0

• Compatible fluorophores: yellow to far-red

Validated fluorophores include JF 549, DL 550, CF 555, CF 568, AF 568, JF 646, AF 647, CF 647, Atto 647N, DL 650, CF 680 & Cy5

• Multicolor imaging: +
• Blinking stability (after mounting): up to 2 months
• Blinking events: +

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Everspark 2.0

• Compatible fluorophores: green, yellow & far-red

Validated fluorophores include AF 488, MemBright 488, FITC, FAM, Spy555, CF 568 & AF 647

• Multicolor imaging : +++
• Blinking stability (after mounting): up to 3.5 months
• Blinking events: +++

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Kit description:

• 10 vials with 450 µL of Everspark buffer at 100 mM MEA in Tris.
• 1 vial per experiment.

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Vials lifetime: Up to 6 months in a closed pouch (eg unmounted).

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Laser requirements: for green fluorophore imaging (Everspark 2.0 only), we recommend using a 488 laser >200mW, ideally 500mW for optimal results.

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Additional information:

• Product overview
• Safety Datasheet - Everspark 1.0
• Safety Datasheet - Everspark 2.0
• Certificate of Analysis - Everspark 1.0
• Certificate of Analysis - Everspark 2.0

Publications

Everspark technology has been intially developed by Karine Monier, Arnaud Favier and Christophe Place and published in Scientific Reports:

Provost, A., Rousset, C., Bourdon, L. et al. Innovative particle standards and long-lived imaging for 2D and 3D dSTORM. Sci Rep 9, 17967 (2019). https://doi.org/10.1038/s41598-019-53528-0

Discover three-color nanoscale imaging with Everspark 2.0 by watching this on-demand webinar by Karine Monier.

Publications:

Dia2 formin controls receptor activity by organizing plasma membrane lipid partitioning at the nanoscale

Changting LI, Yannick HAMON, David MAZAUD, Pamela GONZALEZ TRONCOSO, Marie DESSARD, Hai-tao HE, Christophe LAMAZE, and Cedric M. BLOUIN. bioRxiv 2023.12.15.571857; doi: https://doi.org/10.1101/2023.12.15.571857

Nanoscale engagement and clusterization of Programmed death ligand 1 (PD-L1) in the membrane lipid rafts of Non-Small Cell Lung Cancer cells

Martina Ruglioni, Simone Civita, Tiziano Salvadori, Sofia Cristiani, Vittoria Carnicelli, Serena Barachini, Iacopo Petrini, Irene Nepita, Marco Castello, Alberto Diaspro, Paolo Bianchini, Barbara Storti, Ranieri Bizzarri, Stefano Fogli and Romano Danesi bioRxiv 2022.08.09.503318; doi: https://doi.org/10.1101/2022.08.09.503318

HIV-1 diverts actin debranching mechanisms for particle assembly and release in CD4 T lymphocytes

Rayane Dibsy, Erwan Bremaud, Johnson Mak, Cyril Favard, Delphine Muriaux

Nat Commun. 2023 Oct 31;14(1):6945. doi: 10.1038/s41467-023-41940-0

Fluorescent Polymer-AS1411-Aptamer Probe for dSTORM Super-Resolution Imaging of Endogenous Nucleolin

Fabre L, Rousset C, Monier K, Da Cruz-Boisson F, Bouvet P, Charreyre MT, Delair T, Fleury E, Favier A. Biomacromolecules. 2022 May 12. doi: 10.1021/acs.biomac.1c01706. PMID: 35549176

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, Seifert U, Cammann C, Wesche J, Steil L, Hentschker C, Gesell-Salazar M, Reisinger E, Beer M, Endlich N, Greinacher A, Völker U. Haematologica. 2022 Apr 1;107(4):947-957. doi: 10.3324/haematol.2021.280154. PMID: 35045692

Metabolic biorthogonal labeling and dSTORM imaging of peptidoglycan synthesis in Streptococcus pneumoniae

Jennyfer Trouve, Oleksandr Glushonkov and Cecile Morlot

Star Protocols, December 13, 2021. doi: 10.1016/j.xpro.2021.101006

Insights in ChAdOx1 nCoV-19 vaccine-induced immune thrombotic thrombocytopenia.

Greinacher A, Selleng K, Palankar R, Wesche J, Handtke S, Wolff M, Aurich K, Lalk M, Methling K, Völker U, Hentschker C, Michalik S, Steil L, Reder A, Schönborn L, Beer M, Franzke K, Büttner A, Fehse B, Stavrou EX, Rangaswamy C, Mailer RK, Englert H, Frye M, Thiele T, Kochanek S, Krutzke L, Siegerist F, Endlich N, Warkentin TE, Renné T.

Blood. 2021 Dec 2;138(22):2256-2268. doi: 10.1182/blood.2021013231. PMID: 34587242

Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres.

Szikora S, Novák T, Gajdos T, Erdélyi M, Mihály J. Bio Protoc. 2020 Jun 20;10(12):e3654. doi: 10.21769/BioProtoc.3654. eCollection 2020 Jun 20. PMID: 33659324

Identification of IQCH as a calmodulin-associated protein required for sperm motility in humans

Cavarocchi E, Sayou C, Lorès P, Cazin C, Stouvenel L, El Khouri E, Coutton C, Kherraf ZE, Patrat C, Govin J, Thierry-Mieg N, Whitfield M, Ray PF, Dulioust E, Touré A. iScience. 2023 Jul 10;26(8):107354. doi: 10.1016/j.isci.2023.107354.

PGE2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function

Morotti M, Grimm AJ, Hope HC, Arnaud M, Desbuisson M, Rayroux N, Barras D, Masid M, Murgues B, Chap BS, Ongaro M, Rota IA, Ronet C, Minasyan A, Chiffelle J, Lacher SB, Bobisse S, Murgues C, Ghisoni E, Ouchen K, Bou Mjahed R, Benedetti F, Abdellaoui N, Turrini R, Gannon PO, Zaman K, Mathevet P, Lelievre L, Crespo I, Conrad M, Verdeil G, Kandalaft LE, Dagher J, Corria-Osorio J, Doucey MA, Ho PC, Harari A, Vannini N, Böttcher JP, Dangaj Laniti D, Coukos G. PGE2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function. Nature. 2024 May;629(8011):426-434. doi: 10.1038/s41586-024-07352-w. Epub 2024 Apr 24. PMID: 38658764; PMCID: PMC11078736.

Cis-B7:CD28 Interactions at Invaginated Synaptic Membranes Activate CD28 and Promote CD8+ T Cell Function and Anti-Tumor Immunity

Y. Zhao, C. Caron, Y.-Y. Chan, C. K. Lee, X. Xu,J.Zhang, T. Masubuchi, C. Wu, J.D. Bui, E. Hui. Cis-B7:CD28 Interactions at Invaginated Synaptic Membranes Activate CD28 and Promote CD8+ T Cell Function and Anti-Tumor Immunity. Immunity. 2023 June 13; 56(6): 1187–1203.e12. doi:10.1016/j.immuni.2023.04.005.

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