ION VITAL – MitoVolt [5100]_ION Biosciences - 코아사이언스

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ION VITAL – MitoVolt

Cat.# 5100 / Size: 1 kit

Categories:Cell Health, Mitochondrial Membrane PotentialSynonyms:calcein, etdh, ION Vital – MitoVolt, membrane potential, mito volt, mitochodria, mitovolt, vital

Mitochondrial membrane potential assay kit that uses JC-10, a dye that accumulates in polarized mitochondria where it emits a red-shifted fluorescence, that is more soluble than JC-1. 5 plate.

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Product Description

JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence. JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Mitochondrial depolarization, a key marker of cellular apoptosis, results in a loss of JC-10 accumulation and a reversal to its monomeric form. This reversible behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.

ION Vital – MitoVolt can be used to monitor and/or visualize mitochondrial membrane potential as a static endpoint or in real-time. When following our protocol, ION Vital – MitoVolt provides enough reagents to make 25 mL of working solution, enough for five 96-well plates or 500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations and assay volumes for your application.

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Specifications

Excitation: 490 nm, 540 nm

Emission: 525 nm, 590 nm

MW: ~600 g/mol

Solubility: H2O/DMSO

Purity: >98%

Filter Set: GFP/FITC,

PI/Texas Red Storage: -20°C

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Documentation

Protocol

SDS

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LEARN MORE ABOUT CELL HEALTH INDICATORS AND ASSAYS

 

References

Cossarizza A, Salvioli S. Flow cytometric analysis of mitochondrial membrane potential using JC-1. Curr Protoc Cytom. (2001). Chapter 9:Unit 9.14. (JC-1 protocol)

Donaghy L, et. al. Reactive oxygen species in unstimulated hemocytes of the pacific oyster Crassostrea gigas: a mitochondrial involvement. PLoS One. (2012). 7(10).

Chen CC, Hsieh DS, Huang KJ, et al. Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells. Drug Des Devel Ther. (2014). 8:459-474

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