[Cell culture] Stampwell V - 3D cell imaging [GRE-MOU-V300, GRE-MOU-V500]_Idylle - 코아사이언스

 

Stampwell V - 3D cell imaging

Mold agarose wells to parallelize spheroid and organoid imaging

 

Cat.#                          Shape

GRE-MOU-V300        V300

GRE-MOU-V500        V500

 

Stampwell stamps imprint arrays of wells in hydrogels in just one time. Nothing more to do than load your samples into the wells and image. The Stampwell shapes have been optimized for the imaging of spheroids and organoids.

 

V Shape 300µ:

. Number of pins: 42

. Depth of the well: 1 mm

. Well bottom diameter: 300µm - Shape: circular

. Well upper side: 1mm*0.5mm - Shape: rectangular

 

V Shape 500µ:

. Number of pins: 42

. Shape of the pins: V

. Depth of the well: 1 mm

. Well bottom diameter: 500µm - Shape: circular

. Well upper side: 1mm*0.5mm - Shape: rectangular

Additional resources:

• Product overview
• More information on the Datasheet.

​Results

hiPSCs cyst in alginate capsule in a V-shape well made with Stampwell

scale bar: 500µm

Image credit: Gaëlle Recher - Bordeaux

V-shape imprinted wells

Filled with a single cell-loaded alginate capsule: corresponding imaged with a stereomicroscope and stitched. Gel pad is made of 2% agarose. (Scale bar: 2mm)

Image credit: Gaëlle Recher - Bordeaux

Protocol

How to use Stampwell:

• Read the full protocol: Stampwell Protocol
• Video protocol: How to use Stampwell

Protocol overview

1. Pour liquid agarose (or other hydrogel)

2. Place the stamp

3. Reticulate the hydrogel

4. Remove the stamp

 

FAQ

Find below advice & tips for Stampwell use:

• Stampwell Frequently asked questions

Publications

Original publication:

Stampwell has been originally developed by Gaelle Recher and published in Scientific Reports:

Alessandri K, Andrique L, Feyeux M, Bikfalvi A, Nassoy P, Recher G. All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide. Sci Rep. 2017 Feb 10;7:42378. doi: 10.1038/srep42378. PMID: 28186188; PMCID: PMC5301227.

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Citations:

Merdrignac C, Clément AE, Montfort J, Murat F, Bobe J. auts2 Features and Expression Are Highly Conserved during Evolution Despite Different Evolutionary Fates Following Whole Genome Duplication. Cells. 2022 Aug 30;11(17):2694. doi: 10.3390/cells11172694. PMID: 36078102; PMCID: PMC9454499.

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Sahai-Hernandez, P., Pouget, C., Eyal, S., Svoboda, O., Chacon, J., Grimm, L., Gjøen, T., & Traver, D. (2023). Dermomyotome-derived endothelial cells migrate to the dorsal aorta to support hematopoietic stem cell emergence. eLife, 12, e58300. https://doi.org/10.7554/eLife.58300

 

*본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

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