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Trichinella spiralis & Trichinella britovi qPCR Kit

Cat.# MD06611 / Size: 100 reactions

Manual / MSDS

Trichinella spiralis & Trichinella britovi qPCR Kit is designed for the in vitro detection of T. spiralis and T. britovi genomes.

Trichinella spiralis and Trichinella britovi are two viviparous nematode parasites of the genus Trichinella. T. spiralis and T. britovi are the causative agents of trichinellosis (trichinosis) that can occur in rodents, pigs, bears, hyenas and humans. The first clinical symptoms are gastrointestinal and usually occur after 1-2 days of consuming raw or undercooked meat from an infected animal.

Trichinella spiralis & Trichinella britovi qPCR Kit is designed for the in vitro detection of T. spiralis and T. britovi genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to T. spiralis and T. britovi. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of T. spiralis and T. britovi genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

Shipping Conditions
Room Temperature
Storage Conditions
-85 °C to -15 °C
Format
Lyophilized
Product Category
Predesigned qPCR assays
Compatibility
All standard Real-time thermal cyclers
Target Species
Trichinella spiralis & Trichinella britovi
Sample Material
DNA
Protocol time
Approx. 60 min (from extracted NA to result)
Number of targets
Triplex
Internal Control
No
Features
Free automatic data analyzer (Fastfinder)
Detection Method
Probe Based

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

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