[Veterinary & Agriculture][소 파라인플루엔자 바이러스 3 검출 RT-qPCR 키트] Bovine Parainfluenza virus 3 RT-qPCR Kit [MD06021]_NZYTech - 코아사이언스

 

Bovine Parainfluenza virus 3 RT-qPCR Kit

Cat.# MD06021 / Size: 100 reactions

Manual / MSDS

Bovine Parainfluenza virus 3 RT-qPCR Kit is designed for the in vitro detection of BPI-3 genomes.

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Bovine Parainfluenza virus 3 (BPI-3) is a negative single-stranded RNA virus that belongs to Respirovirus genus within the Paramyxoviridae family. BPI-3 is a worldwide recognized endemic agent in the cattle. Clinical symptoms due to BPI-3 infection can be extremely variable, going from asymptomatic infections to severe respiratory disease and pneumonia (cough, pyrexia and nasal discharge). Clinical disease usually develops in young calves with low level of maternal passive antibodies or in animals under stress conditions.

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Bovine Parainfluenza virus 3 RT-qPCR Kit is designed for the in vitro detection of BPI-3 genotypes A, B and C. The kit is built to have the broadest possible detection profile whilst remaining specific to BPI-3. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of BPI-3 genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme One-step RT-qPCR Master Mix (2x)

- RT-qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- RNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Bovine Parainfluenza virus 3
Sample Material	RNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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