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[Food & Water Safety][레지오넬라 뉴모필라 검출 qPCR 키트] Legionella pneumophila qPCR Kit [MD05281]_NZYTech - 코아사이언스
코피디 2023. 8. 4. 11:40Legionella pneumophila qPCR Kit
Cat.# MD05281 / Size: 100 reactions
Legionella pneumophila qPCR Kit is designed for the in vitro detection of L. pneumophila genomes.
Legionella pneumophila is a Gram-negative bacterium of the genus Legionella. In nature, L. pneumophila infects freshwater and also soil amoebae of the genera Acanthamoeba and Naegleria. L. pneumophila is the primary human pathogenic bacterium in this group and is the causative agent of Legionnaires' disease, also known as legionellosis.
Legionella pneumophila qPCR Kit is designed for the in vitro detection of L. pneumophila genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to L. pneumophila. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of L. pneumophila genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. Real-time PCR detection methods have been used in several studies to identify L. pneumophila genomes (Donohue M.J. 2021).
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Components:
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
Shipping Conditions
|
Room Temperature
|
Storage Conditions
|
-85 °C to -15 °C
|
Format
|
Lyophilized
|
Product Category
|
Predesigned qPCR assays
|
Compatibility
|
All standard Real-time thermal cyclers
|
Target Species
|
Legionella pneumophila
|
Sample Material
|
DNA
|
Protocol time
|
Approx. 60 min (from extracted NA to result)
|
Number of targets
|
Duplex
|
Internal Control
|
No
|
Features
|
Free automatic data analyzer (Fastfinder)
|
Detection Method
|
Probe Based
|
* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.
코아사이언스 coresciences NZYTech, Lda. genes & enzymes korea 한국 molecular biology 분자생물학 enzymes & proteins 효소 단백질 assay kits 분석 측정 테스트 키트
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