[Food & Water Safety][마이코박테리아 검출 qPCR 키트] Mycobacterium spp qPCR Kit [MD05071]_NZYTech - 코아사이언스

 

Mycobacterium spp qPCR Kit

Cat.# MD05071 / Size: 100 reactions

Manual / MSDS

Mycobacterium spp qPCR Kit is designed for the in vitro detection of Mycobacterium spp. genomes.

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Mycobacterium is a genus of Gram-negative bacteria in the phylum Actinomycetota. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (Mycobacterium tuberculosis) and leprosy (Mycobacterium leprae) in humans.

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Mycobacterium spp qPCR Kit is designed for the in vitro detection of Mycobacterium spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Mycobacterium genus. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. The primers and probe sequences have very high (>95%) homology with a broad range of Mycobacterium spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. However, the detection of all Mycobacterium strains cannot be guaranteed. Moreover, in silico analysis showed a possible cross reactivity with a number of species belonging to the genera Rhodococcus and Nocardia. Real-time PCR detection methods have been used in several studies to identify Mycobacterium spp. genomes (Radomski N. et al 2010).

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech(see Molecular Diagnostics Homepage. The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Mycobacterium species
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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