[Dyes & Labeling/Fluorescent Dyes/Fluorescein Derivatives/Non-Activated] 5/6-Carboxyfluorescein, mixed isomers (5/6-FAM) (99+%) [F-0101][CAS no. 72088-94-9]_emp Biotech - 코아사이언스

 

5/6-Carboxyfluorescein, mixed isomers (5/6-FAM) (99+%)

Cat.# F-0101-D100.0-001 / Size: 100mg

Cat.# F-0101-E001.0-001 / Size: 1g

High purity, non-activated fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 496 nm, Em: 517 nm).

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Properties

CAS-Number:	72088-94-9
Molecular Weight:	376,32
Empirical Formula:	C21H12O7
Absorption [nm]:	493 ± 3
Emission [nm]:	513 ± 3
ε [M-1 cm-1]:	>81 000
Excitation [nm]:	493 494
Buffer System for Fluorescence Readings:	T&S Buffer, pH 9
Solubility:	pH > 6, DMF, DMSO
Appearance (colour):	orange
Appearance (form):	solid (powder)
Storage:	RT! Keep dry! Keep away from light, especially in solution!
Trade name:	5/6-Carboxyfluorescein; 5/6-FAM; 5-(and 6)-carboxyfluorescein, mixed isomers
Reactive Moiety:	free carboxylic acid

Description

This highly purified mixed isomer fluorescein derivatization reagent is widely used for covalent labeling of proteins and alkylamino modified nucleic acids.

Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.

In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. In addition, fluorescein's protein conjugates are not inordinately susceptible to precipitation.

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5/6-FAM

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