[Dyes & Labeling/Fluorescent Dyes/Fluorescein Derivatives/Non-Activated] 5/6-Carboxytetramethylrhodamine, mixed isomers (5/6-TAMRA) [F-0102][CAS no. 98181-63-6]_emp Biotech - 코아사이언스

 

5/6-Carboxytetramethylrhodamine, mixed isomers (5/6-TAMRA)

Cat.# F-0102-D025.0-001 / Size: 25mg

Cat.# F-0102-D100.0-001 / Size: 100mg

Cat.# F-0102-E001.0-001 / Size: 1g

Cat.# F-0102-E005.0-001 / Size: 5g

Non-activated rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 548 nm, Em: 569 nm).

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Properties

CAS-Number:	98181-63-6
Molecular Weight:	466,9
Empirical Formula:	C25H23ClN2O5
Absorption [nm]:	548 ± 3
Emission [nm]:	570 ± 3
ε [M-1 cm-1]:	>78 000
Buffer System for Fluorescence Readings:	PBS, pH 7.4
Solubility:	Methanol, DMF, DMSO
Appearance (colour):	violett
Appearance (form):	solid (powder)
Reactive Moiety:	free carboxylic acid

Description

The non-activated carboxytetramethyl rhodamine derivative is used as a red fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids.

The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546 nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543 nm spectral line of the green He-Ne laser.

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5/6-TAMRA

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