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Dicarboxymethylene Blue (DCMB)

Cat.# F-0201-D005.0-001 / Size: 5mg

Non-activated methylene blue redox marker for electro-active labeling, designed especially for labeling of biomolecules.

Properties

Molecular Weight:
527,98
Empirical Formula:
C22H26N3O8SCl
Absorption [nm]:
669 ± 3
Emission [nm]:
686 ± 3
ε [M-1 cm-1]:
>53 000
Buffer System for Fluorescence Readings:
TE Buffer, pH 7.6
Solubility:
Acetonitrile
Appearance (colour):
dark blue
Appearance (form):
solid (powder)
Storage:
- 20 °C. Keep dry! Keep away from light, especially in solution!
Trade name:
Dicarboxymethylene blue
Reactive Moiety:
free carboxylic acid

Description

This non-activated methylene blue derivative is used as an electroactive labeling reagent for covalent attachment to proteins and alkylamino modified nucleic acids. Dicarboxymethylene blue contains two carboxylic acid functional groups. In addition to its relatively high absorption, DCMB has excellent and validated redox properties and is a good generator for singlet oxygen, making it to a particularly suitable label for electrochemical and photosensitzer applications.

CAS: none known

Formal Electrode Potential vs. Ag/AgCl (1 M KCl): Ef = -0.216 V

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