[암/종양 연구 관련 라미닌 동형(isoform) 특이적 단클론 항체] 4 unique monoclonal antibodies for cancer-related anti-laminin [FDV-0023, FDV-0024, FDV-0025, FDV-0026]_Funakoshi - 코아사이언스

4 unique monoclonal antibodies for cancer-related anti-laminin
Useful monoclonal antibodies for the research of cancer pathology

Laminins are major extracellular matrix proteins containing over 15 members. Laminins are large hetero-trimers consisting of 5 alpha chains, 3 beta chains, and 3 gamma chains. Due to its structural varieties, it has been challenging to detect each laminin isoform by conventional antibodies.

 

Funakoshi commercializes 4 unique antibodies, originally developed by Dr. Kaoru Miyazaki,

Professor Emeritus of Yokohama City University in Japan.

Our antibodies can separately recognize specific isoforms or structures of laminin. 

 

Fig.1 Structure of Laminin 3A32, 3B32 and γ2pf fragment with epitopes of 3 clones (F7, BG5 andP2H).

 

Features 

■ BG5/F7 : For laminin alpha-3 chains

- Laminin alpha 3 chain, a major component of Laminin-332, has two splicing variants, alpha 3A and alpha 3B
- Clone BG5 is specific antibody for alpha 3A
- Clone F7 is the world first commercially available antibody for alpha 3B

 

 

■ P2H : For laminin gamma-2 chain

- Laminin gamma2, an only component of Laminin-332, is a marker gene of invasive cancer tissue
- Laminin gamma-2 chain is activated via proteolytical cleavage at N-terminal
- N-terminal proteolytic fragment of gamma-2, called γ2pf, is highly accumulated into cytosol under malignant cancer
- Clone P2H is the world first antibody for γ2pf

 

 

■ 12D : For laminin-511

- There are two similar isoforms, laminin-511 and laminin-521 and it is difficult for conventional antibodies

 such as anti-alpha 5 chain to separate them.
- Clone 12D specifically react with trimeric structure of laminin-511, not react with each components

  alpha 5, beta 1, gamma 1 and similar isoform laminin-511.  

 

<Note>

Laminin 511 is used as a culture substrate for ES and iPS cells. (See our iMatrix-511 / EasyiMatrix-511). 

 

Fig.2 Structure of Laminin-511

 

Outline of four clones 

Description	Anti-Laminin alpha 3A	Anti-Laminin alpha 3B	Anti-Laminin gamma 2 N-terminal Fragment	Anti-Laminin-511
Clone	BG5	F7	P2H	12D
Specificity	Laminin alpha 3A *1	Laminin alpha 3B	Laminin gamma 2  Domain V-NE2	Laminin-511
Host and Type	Mouse Monoclonal
Subclass	IgG2a	IgG1	IgG2a	IgG2b
Reactivity	Human	Human	Human, Mouse	Human
Form	Mouse Ascites  (Unpurifed)
Application	ELISA, IHC, IP, WB		IHC (frozen & paraffin  sections), WB	IHC (frozen), IP, WB  （Non-reducing) *2
Size	100 uL
Storage	-20℃ *3
Cat.#	FDV-0024	FDV-0023	FDV-0025	FDV-0026

 

*1 Clone BG5 only detects alpha 3A in IHC. However, this clone may react with alpha 3B in ELISA, IP and Western Blot.

*2 Clone 12D can be only used for IHC (Frozen) and under non-reducing condition for Western Blot.

*3 Non-preservative added. So these 4 clones can be degraded at 4℃. We recommend -20℃ storage.

 

Data Examples 

Fig.3 IHC image of skin cancer tissue in clone BG5 and F7

Left : Clone BG5, Right : Clone F7

Both clones detectedbasal membrane like structure around the tumor but F7 clone also detected vascular basement membrane (arrow heads).

 

Fig.4 IHC image of tissues in clone P2H

Upper images : Normal mammary gland

Lower images: Breast cancer

P2H stains basement membranes surrouding mammary glands in normal tisue, whereas in a breast cancer tissue cytoplasmic accumulation of γ2pf orits fragments is detected.

 

Fig.5 IHC image ofbreast cancer tissue in clone 12D

A : Vascular basement membrane

B: Mammary gland basement membrane

C : Basement membrane-like structures surrounding tumor cells

Fig.6 Specificity of clone 12D against laminin isoforms

Seven recombinant human laminins were coated on a multi-well plate and detected by clone 12D. The data shows clone 12D specifically reacts with Laminin 511.

 

Ordering Information 

Product	Size	Cat.#	Storage
Anti-Laminin alpha 3B, Human, Mouse-Mono (F7)	100 μL	FDV-0023	-20℃
Anti-Laminin alpha 3A, Human, Mouse-Mono (BG5)	100 μL	FDV-0024	-20℃
Anti-Laminin gamma 2 N-terminal fragment, Human, Mouse-Mono (P2H)	100 μL	FDV-0025	-20℃
Anti-Laminin-511, Human, Mouse-Mono (12D)	100 μL	FDV-0026	-20℃

 

Citations and References

1. Kariya, Y., et al., J. Mol. Histol., 39, 435-446 (2008) (Clone BG5/F7)
2. Mori, T., et al., Cancer Sci., 102, 1095-1100 (2011) (Clone F7)
3. Miyazaki, K., et al., Cancer Sci., 107, 1909-1918 (2016) (Clone BG5, P2H)
4. Komiya, E., et al., Cancer Med., 3, 537-549 (2014) (Clone 12D)

coresciences 코아사이언스 funakoshi  bioacademia 항체 한국 대리점 기저막 주요구성분 세포접착성 당단백질  Laminin isoform라미닌 동형 단백질