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Transmissible Gastroenteritis virus RT-qPCR Kit

Cat.# MD06601 / Size: 100 reactions

Manual / MSDS

Transmissible Gastroenteritis virus RT-qPCR Kit is designed for the in vitro detection of TGEV genomes.

Transmissible gastroenteritis virus (TGEV) is a positive single-stranded RNA virus that belongs to the genus Alphacoronavirus within the family Coronaviridae. TGEV is a coronavirus, which infects pigs of all ages, and leads to an acute, rapidly spreading disease called transmissible gastroenteritis (TGE), characterized by diarrhea and vomiting.

Transmissible Gastroenteritis virus RT-qPCR Kit is designed for the in vitro detection of TGEV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to TGEV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of TGEV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. This kit does not detect Porcine Respiratory Coronavirus (PRCV). Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

Components:

- Lyo NZYSupreme One-step RT-qPCR Master Mix (2x)

- RT-qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- RNA Internal Extraction Control (IEC)

 

Shipping Conditions
Room Temperature
Storage Conditions
-85 °C to -15 °C
Format
Lyophilized
Product Category
Predesigned qPCR assays
Compatibility
All standard Real-time thermal cyclers
Target Species
Transmissible Gastroenteritis virus
Sample Material
RNA
Protocol time
Approx. 60 min (from extracted NA to result)
Number of targets
Duplex
Internal Control
No
Features
Free automatic data analyzer (Fastfinder)
Detection Method
Probe Based

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

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