[엔테로코커스 패칼리스 검출 qPCR 키트] Enterococcus faecalis qPCR Kit [MD05001]_NZYTech - 코아사이언스

 

Enterococcus faecalis qPCR Kit

Cat.# MD05001 / Size: 100 reactions

Manual / MSDS

Enterococcus faecalis qPCR Kit is designed for the in vitro detection of E. faecalis genomes.

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Enterococcus faecalis is a Gram-positive, commensal bacterium inhabiting the gastrointestinal tracts of humans. Like other species in the genus Enterococcus, E. faecalis is found in healthy humans and can be used as a probiotic. However, in certain situations E. faecalis acts as an opportunistic pathogen causing life-threatening infections, especially in the nosocomial environment, where the naturally high levels of antibiotic resistance found in E. faecalis contribute to its pathogenicity.

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Enterococcus faecalis qPCR Kit is designed for the in vitro detection of E. faecalis genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to E. faecalis. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of E. faecalis genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. Real-time PCR detection methods have been used in several studies to identify E. faecalis genomes (Teng L. et al 2001).

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Enterococcus faecalis
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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