[Food & Water Safety][살모넬라 검출 qPCR 키트] Salmonella spp. qPCR Kit [MD05421]_NZYTech - 코아사이언스

Salmonella spp. qPCR Kit

Cat.# MD05421 / Size: 100 reactions

Manual

 

Salmonella spp. qPCR Kit is designed for the in vitro detection of Salmonella spp. genomes.

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Salmonella is a rod-shape Gram-negative bacterium that belongs to the family of Enterobacteriaceae. Salmonella species are intracellular food-borne pathogens that cause infections due to ingestion of food contaminated by animal or human feces.

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Salmonella spp. qPCR Kit is designed for the in vitro detection of Salmonella spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Salmonella spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of Salmonella spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. Real-time PCR detection methods have been used in several studies to identify Salmonella spp. genomes (Kasturi K.M. et al 2001).

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Salmonella spp.
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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