[Cell isolation & staining] CD3 Fab-TACS® Agarose Column Starter Kit, mouse [6-3304-002]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스

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IBA Lifesciences GmbH 수입/공급 업체 코아사이언스 입니다.

 

자세한 상품 정보 및 관련 문의는 전화 02-858-0328 또는 info@coresciences.co.kr로 문의주시기 바랍니다.

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CD3 Fab-TACS® Agarose Column Starter Kit, mouse

Label-free isolation of T cells from murine splenocytes

Cat.# 6-3304-002 / Size: Reagents for 2 isolations

Protocol with Data Sheet Information

Label-free Cell Isolation from Splenocytes with Strep-Tactin® TACS Agarose

Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD3 Fab-TACS® Agarose Column Starter Kit allows label-free isolation of murine CD3+ T cells from splenocytes or other cell suspensions. CD3 is a co-receptor that associates with the T cell receptor and plays a role in T cell activation.

Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.

 

Advantages:
• Highly pure cells
• Reversible binding to the target
• Highly viable, non-activated cells
• Label-free cells due to low affinity nanobodies

 

The kit contains:
• Strep-Tactin® TACS Agarose columns, 0.3 ml (2x)
• CD3 Fab-Strep, mouse
• 10x Buffer CI
• Biotin stock solution
• TACS Column Adapter

Specifications

Capacity: 1 x 10^9 total/1x10^8 target cells per column

 

Shipping information

Storage: 2-8 °C

Stability: 6 months after shipping

Shipping: Room temperature

 

추천 상품 

[가스 투과성 세포 배양 백] NIPRO Cell Culture Bags [87-352, 87-302] & Connecting Tube [87-360]

[마이크로플레이트 웰에서 효과적인 세포 회수/소형 셀 스크래퍼] Mini Cell Scraper: Best size for 24, 48 and 96 well culture plates [MCS-200]

 

미니 셀 스트래이너/미니 셀 스트레이너/Single cell 분리] Mini Cell Strainers for 5 ml sampling tubes and 15 ml conical tubes [HT-AMS-12502, HT-AMS-14002, HT-AMS-17002] & Mini Cell Strainer II for 1.5 mL/2.0 mL tube and 5 ml sampling tubes [HT-AMS-04002, HT-AMS-04008]

코아사이언스 coresciences IBA Lifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation & expansion