[Cell isolation & staining] CD3/CD45RO Fab-TACS® Magnetic Microbead Starter Kit, human [6-8000-117]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스

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IBA Lifesciences GmbH 수입/공급 업체 코아사이언스 입니다.

 

자세한 상품 정보 및 관련 문의는 전화 02-858-0328 또는 info@coresciences.co.kr로 문의주시기 바랍니다.

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CD3/CD45RO Fab-TACS® Magnetic Microbead Starter Kit, human

Label-free isolation of memory T cells from PBMCs

Cat.# 6-8000-117 / Size: Reagents for 1 x 10^8 total cells

Protocol with Data Sheet Information

Label-free Cell Isolation from PBMCs with Strep-Tactin® Magnetic Microbeads

Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD3/CD45RO Fab-TACS® Magnetic Microbead Starter Kit allows label-free isolation of memory T cells (CD3+CD45RO+) from peripheral blood mononuclear cells (PBMCs). CD45RO is an isoform of tyrosine phosphatase CD45 (leukocyte common antigen) that is expressed on activated and memory T cells, B cell subsets, neutrophils as well as activated monocytes and macrophages.

Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) are incubated with Strep-Tactin® Magnetic Microbeads to form a complex that stably binds to the cell surface. After specific labeling, target cells are bound to a magnet and non-target cells efficiently washed away. In a final step, the addition of biotin causes the release of the isolated cells from magnetic microbeads. The Fab-Streps self-dissociate from the cell surface due to their low affinity. After removing biotin by washing, the isolated cells are ready for further positive selection or depletion steps or can directly be used for further downstream applications.

 

Advantages:
• High purity of > 90%
• Scalable reagents
• Label-free cells due to low affinity Fab fragments
• Non-activated cells

 

The kit contains:
• Strep-Tactin® Magnetic Microbeads
• CD3 Fab-Strep, human

• CD45RO Fab-Strep, human

• 10x Buffer CI

• Biotin stock solution

 

Specifications

Capacity: 1 x 10^8 total cells

 

Shipping information

Storage: 2-8 °C

Stability: 6 months after shipping

Shipping: Blue ice

 

추천 상품 

[가스 투과성 세포 배양 백] NIPRO Cell Culture Bags [87-352, 87-302] & Connecting Tube [87-360]

[마이크로플레이트 웰에서 효과적인 세포 회수/소형 셀 스크래퍼] Mini Cell Scraper: Best size for 24, 48 and 96 well culture plates [MCS-200]

 

미니 셀 스트래이너/미니 셀 스트레이너/Single cell 분리] Mini Cell Strainers for 5 ml sampling tubes and 15 ml conical tubes [HT-AMS-12502, HT-AMS-14002, HT-AMS-17002] & Mini Cell Strainer II for 1.5 mL/2.0 mL tube and 5 ml sampling tubes [HT-AMS-04002, HT-AMS-04008]

코아사이언스 coresciences IBA Lifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation & expansion