[Cell isolation & staining] CD3 Fab-Strep, mouse [6-8504-150]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스

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CD3 Fab-Strep, mouse

Low affinity Fab fragment fused to a Twin-Strep-tag® and specific for murine CD3

Cat.# 6-8504-150 / Size: 50ug

Data Sheet

Protocol for Fab-TACS® fluorescent staining

CD3 Fab-Strep is a recombinantly expressed low affinity Fab fragment fused to a Twin-Strep-tag®. Fab-Streps were developed for Traceless Affinity Cell Selection (Fab-TACS®), which is based on our Strep-tag® technology. Due to their low affinity for the target, an increase in avidity is required to ensure sufficient binding to the cell surface. Combining Fab-Streps with a Strep-Tactin® backbone leads to the formation of complexes that stably bind to cells.

Depending on the Strep-Tactin® backbone, the following applications are possible:
• Fluorescent cell staining & sorting: Fab-Streps are combined with fluorophore-conjugated Strep-Tactin®.
• Affinity chromatographic cell isolation: Fab-Streps are loaded in Strep-Tactin® TACS Agarose columns.

By addition of biotin, the labeling reagents dissociate mildly and rapidly from the selected target cells in all applications, yielding minimally manipulated cells for unbiased further use.

Specifications

Endotoxin Level: ≤ 0.1 EU/µg by LAL test

Form: Lyophilized

Possible Application: Fluorescent cell staining & sorting, affinity chromatographic cell isolation

Purity: ≥ 90%

Reconstitution: Reconstitute in 1 ml buffer, e.g. 1x PBS containing 1 mM EDTA and 0.5% BSA, for a final concentration of 50 µg/ml.

Specificity: Murine CD3

 

Shipping information

Storage: 2-8 °C; reconstituted: -80 °C

Stability: 6 months after shipping

Shipping: Room temperature or blue ice

 

추천 상품 

[가스 투과성 세포 배양 백] NIPRO Cell Culture Bags [87-352, 87-302] & Connecting Tube [87-360]

[마이크로플레이트 웰에서 효과적인 세포 회수/소형 셀 스크래퍼] Mini Cell Scraper: Best size for 24, 48 and 96 well culture plates [MCS-200]

 

미니 셀 스트래이너/미니 셀 스트레이너/Single cell 분리] Mini Cell Strainers for 5 ml sampling tubes and 15 ml conical tubes [HT-AMS-12502, HT-AMS-14002, HT-AMS-17002] & Mini Cell Strainer II for 1.5 mL/2.0 mL tube and 5 ml sampling tubes [HT-AMS-04002, HT-AMS-04008]

코아사이언스 coresciences IBA Lifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation