Nano-TACS®- Traceless Affinity Cell Selection -is a positive cell isolation approach based on ourStrep-tag®technology. CD19 Nano-TACS®Agarose Column Starter Kit allows label-free isolation of murine CD19+B cells from splenocytes or other cell suspensions. CD19 is an adaptor protein that is involved in signaling pathways.
Strep-Tactin®TACS Agarose columns are filled with Strep-Tactin®coated agarose macrobeads developed for cell isolation. Low affinity nanobodies fused to a Twin-Strep-tag®(Nano-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Nano-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Nano-Streps. After elution, the Nano-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.
Advantages: • Highly pure cells • Reversible binding to the target • Highly viable, non-activated cells • Label-free cells due to low affinity nanobodies
코아사이언스 coresciences IBA Lifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation & expansion
