[Antigen-specific T cell isolation & staining] Strep-Tactin® TACS Agarose Column [6-6310-300, 6-6310-001]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스

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IBA Lifesciences GmbH 수입/공급 업체 코아사이언스 입니다.

 

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Strep-Tactin® TACS Agarose Column

Gravity flow column suitable for traceless affinity cell and exosome isolation

Cat.# 6-6310-300 / Size: 0.3ml

Cat.# 6-6310-001 / Size: 1ml

Data Sheet

Protocol for Fab-TACS® affinity chromatographic exosome isolation (1 ml column)

Label-free Cell Isolation from Blood with Strep-Tactin® TACS Agarose

Label-free Cell Isolation from Splenocytes with Strep-Tactin® TACS Agarose

Strep-Tactin® TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the use for cell isolation and depletion approaches. They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS®/Nano-TACS®). In this approach, Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin® on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin® Agarose by biotin addition. The cells are eluted and ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS® for exosomes). Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding). This makes Strep-Tactin® TACS Agarose columns also suitable for negative cell selection or depletion approaches.

Specifications

Form: Pre-packed in buffer

Possible Application: Affinity chromatographic cell or exosome isolation

Support: Crosslinked agarose

 

Shipping information

Storage: 2-8 °C

Stability: 6 months after shipping

Shipping: Room temperature

 

추천 상품 

[가스 투과성 세포 배양 백] NIPRO Cell Culture Bags [87-352, 87-302] & Connecting Tube [87-360]

[마이크로플레이트 웰에서 효과적인 세포 회수/소형 셀 스크래퍼] Mini Cell Scraper: Best size for 24, 48 and 96 well culture plates [MCS-200]

 

미니 셀 스트래이너/미니 셀 스트레이너/Single cell 분리] Mini Cell Strainers for 5 ml sampling tubes and 15 ml conical tubes [HT-AMS-12502, HT-AMS-14002, HT-AMS-17002] & Mini Cell Strainer II for 1.5 mL/2.0 mL tube and 5 ml sampling tubes [HT-AMS-04002, HT-AMS-04008]

코아사이언스 coresciences IBA Lifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation & expansion