[Antigen-specific T cell isolation & staining] Strep-Tactin® Magnetic Microbeads [6-5510-050]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스

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IBA Lifesciences GmbH 수입/공급 업체 코아사이언스 입니다.

 

자세한 상품 정보 및 관련 문의는 전화 02-858-0328 또는 info@coresciences.co.kr로 문의주시기 바랍니다.

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Strep-Tactin® Magnetic Microbeads

Strep-Tactin® multimers conjugated with magnetic microbeads

Cat.# 6-5510-050 / Size: 750ul

Data Sheet

Protocol for MHC I Streptamer® magnetic isolation

Label-free Cell Isolation from PBMCs with Strep-Tactin® Magnetic Microbeads

 

Strep-Tactin® Magnetic Microbeads are microspheres covalently linked to Strep-Tactin®. They are suitable for labeling antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®). To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin®. Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting magnetic complexes can be used to label cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents permits serial positive selection as well as depletion steps and reserves the cells’ full biological activity.

Specifications

Bead Size: 1 - 3 µm

Form: Suspension in DPBS

Possible Application: Cell isolation (magnetic)

Recommended Usage: 15 µl per 1 x 10^7 total cells

 

Shipping information

Storage: 2-8 °C; Do not freeze!

Stability: 6 months after shipping

Shipping: Blue ice

 

추천 상품 

[가스 투과성 세포 배양 백] NIPRO Cell Culture Bags [87-352, 87-302] & Connecting Tube [87-360]

[마이크로플레이트 웰에서 효과적인 세포 회수/소형 셀 스크래퍼] Mini Cell Scraper: Best size for 24, 48 and 96 well culture plates [MCS-200]

 

미니 셀 스트래이너/미니 셀 스트레이너/Single cell 분리] Mini Cell Strainers for 5 ml sampling tubes and 15 ml conical tubes [HT-AMS-12502, HT-AMS-14002, HT-AMS-17002] & Mini Cell Strainer II for 1.5 mL/2.0 mL tube and 5 ml sampling tubes [HT-AMS-04002, HT-AMS-04008]

코아사이언스 coresciences IBA Lifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation & expansion