[Antigen-specific T cell isolation & staining] Strep-Tactin® TACS Agarose [6-6350-002, 6-6350-010, 6-6350-025]_IBA Lifesciences GmbH - 수입/공급 전문 코아사이언스

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Strep-Tactin® TACS Agarose

50% suspension for traceless affinity cell and exosome isolation

Cat.# 6-6350-002 / Size: 4ml

Cat.# 6-6350-010 / Size: 20ml

Cat.# 6-6350-025 / Size: 50ml

Data Sheet

Label-free Cell Isolation from Blood with Strep-Tactin® TACS Agarose

Label-free Cell Isolation from Splenocytes with Strep-Tactin® TACS Agarose

Strep-Tactin® TACS Agarose contains agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the use for cell isolation and depletion approaches. It was developed for column-based Traceless Affinty Cell Selection (Fab-TACS®/Nano-TACS®). In this approach, Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin® on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin® Agarose by biotin addition. The eluted cells are ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS® for exosomes) Alternatively to Fab-Streps/Nano-Streps, it is possible to bind Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding) to this resin. This makes Strep-Tactin® TACS Agarose also suitable for negative cell selection or depletion approaches. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.

Specifications

Biotin Binding Capacity: > 40 nmol/ml resin

Form: 50% suspension

Possible Application: Affinity chromatographic cell or exosome isolation

Support: Crosslinked agarose

 

Shipping information

Storage: 2-8 °C

Stability: 6 months after shipping

Shipping: Room temperature

 

추천 상품 

[가스 투과성 세포 배양 백] NIPRO Cell Culture Bags [87-352, 87-302] & Connecting Tube [87-360]

[마이크로플레이트 웰에서 효과적인 세포 회수/소형 셀 스크래퍼] Mini Cell Scraper: Best size for 24, 48 and 96 well culture plates [MCS-200]

 

미니 셀 스트래이너/미니 셀 스트레이너/Single cell 분리] Mini Cell Strainers for 5 ml sampling tubes and 15 ml conical tubes [HT-AMS-12502, HT-AMS-14002, HT-AMS-17002] & Mini Cell Strainer II for 1.5 mL/2.0 mL tube and 5 ml sampling tubes [HT-AMS-04002, HT-AMS-04008]

코아사이언스 coresciences IBA Lifesciences GmbH 독일 한국 대한민국 cell selection & expansion exosome isolation protein production & assay cell isolation staining antigen specific T cell isolation & staining T cell stimulation & expansion