[Transglutaminases] Human tissue transglutaminase [T022]_ZEDIRA - 코아사이언스

안녕하세요!

ZEDIRA GmbH 수입/공급 업체 코아사이언스 입니다.

 

자세한 상품 정보 및 관련 문의는 전화 02-858-0328 또는 info@coresciences.co.kr로 문의주시기 바랍니다.

감사합니다!

Human tissue transglutaminase

hTG2, rec. produced in insect cells

Cat.# T022 / Size: 250ug, 1mg

Documents: PDS MSDS

Synonym: Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase

Molecular Weight: 78 kDa (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72)

Source: Recombinant produced in insect cells

Purity: > 90 % (visually by SDS-PAGE)

Activation: His6-rhTG2 is a Ca2+-dependent enzyme. Add 10 mM Ca2+ to activate His6-rhTG2.

Activity: > 1500 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rhTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231).

1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].

Appearance: White lyophilized solid.

Reagents: The Transglutaminase is lyophilized from 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 1 mM EDTA, 5 mM DTT. Sample contains maltodextrin.

Reconstitution: Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.

Application: His6-rhTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation.

Storage: Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery is possible at ambient temperature.

Reference(s):

Engstrom et al., Sci. Rep. 2017, 7:77

Hardes et al., Anal. Biochem. 2012, 428:73-80

​

Note

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

​

Related Products

A033: Monoclonal antibody to human TG2 (Catalytic Domain, clone XTG17)

F014: ZediXcite 330/418 Fluorogenic TG2-Assay Kit

Z006: Z-DON-Val-Pro-Leu-OMe, "Z-DON"

​

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences​ ZEDIRA GmbH, Darmstadt, Germany Korea 한국 대리점 distributor 트랜스글루타미나제 트랜스글루타미나아제 트랜스글루타미네이스 트랜스글루타미네이즈 활성 분석 측정 키트