[DNA 단편화 효소] DNA Fragmentation Enzyme [40061, 40062, 40063]_BioDynami - 코아사이언스

DNA Fragmentation Enzyme

Cat.#      Product                                                                  Protocol       MSDS

40061     DNA Fragmentation Enzyme Mix                       download   download

40062    DNA Fragmentation & A-Tailing Enzyme Mix    download   download

40063    DNA Fragmentation & Blunting Enzyme Mix    download    download

 

 

The enzymes were developed for random fragmentation of genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) in a fast and simple way. The DNA fragments contain 5´-phosphates and 3´-hydroxyl groups.

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• Cat. # 40061 DNA Fragmentation Enzyme Mix
• Cat. # 40062 DNA Fragmentation & A-tailing Enzyme Mix
• Cat. # 40063 DNA Fragmentation & Blunting Enzyme Mix

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Enzyme-based fragmentation of DNA is very simple and effective comparing to the mechanical DNA shearing methods. There are several advantages of enzymatic DNA fragmentation, include easy handling of many samples at the same time, and less loss of samples. Moreover, it does not require expensive capital expense for the equipment.

 

 

DNA Fragmentation Enzyme Workflow

The resulting DNA fragment size is inversely correlated with the incubation time of step 1 at 20°C. The fragmented DNA can be used for applications such as Next-Generation Sequencing (NGS). Our Fragmentation Enzyme does not generate detectable sequencing bias. Sequence coverage is also consistent between enzymatic and mechanical fragmentation.

 

DNA fragment size is inversely correlated with the incubation time of step 1 at 20°C.

 

DNA Fragmentation Enzyme Mix (Cat. # 40061)

The enzyme mix was developed for enzymatic fragmentation of genomic DNA with random ends in a single step. The fragmented DNA can be used for applications such as Next-Generation Sequencing (NGS).

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DNA Fragmentation & A-tailing Enzyme Mix (Cat. # 40062)

The enzyme mix can be used for enzymatic fragmentation of genomic DNA and addition of A-tailing at 3’-ends of DNA in a single step. 3’-ends of DNA with A-tailing can be used in many downstream applications in the field of molecular biology such as next generation sequencing, PCR cloning, and TA-ligation etc.

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DNA Fragmentation & Blunting Enzyme Mix (Cat. # 40063)

The enzyme mix was developed to generate random fragmentation of genomic DNA with blunt ends in a single step. The fragmented DNA can be used for applications such as Next-Generation Sequencing (NGS), blunt end ligation and PCR cloning.

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Features

• Fast enzymatic fragmentation: 30-45 minutes
• Simple Procedure: 3 minutes of hands-on time
• Works with both EDTA-free DNA and DNA resuspended in TE buffer
• Ideal for downstream reactions: NGS library prep, PCR cloning, TA-ligation, and blunt end ligation etc.

Comparison of Fragmentation Enzymes

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Related Products

• NGS DNA Fragmentation & Library Prep Kit (illumina)
• NGS DNA Fragmentation & Library Prep Kit (ion torrent)
• NGS DNA Library Prep Kit (Illumina Platform)
• NGS low Input DNA Library Prep Kit (Illumina Platform)

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코아사이언스 coresciences BioDynami Korea distributor 한국 대리점 NGS Library Preparation Targeted Sequencing DNA purification magnetic beads Nucleic acid extrasction DNA RNA Quantification DNA Fragmentation DNA ladder 핵산 분리 정제 추출 시퀀싱 차세대 염기서열 분석 키트 시약 마그네틱 비드