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Plasmid Purification Magnetic Beads (RNA Depletion)

Cat.# 40059S / Size: 40 runs

Cat.# 40059L / Size: 200 runs

Protocol download

MSDS download

 

Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.

 

We have developed a simple reagent to completely remove RNA contamination in the isolated plasmid samples using Solid Phase Reversible Immobilization (SPRI) beads. SPRI beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. Our Plasmid Purification Magnetic Beads (RNA Depletion) combines BioDynami’s proprietary chemistries with the reversible DNA-binding properties of SPRI magnetic beads. The reagent removes RNA and recovers the plasmid in the same step. Moreover, unwanted components such as salts, dNTPs, proteins, enzymes, and other impurities can also be removed simultaneously.

Plasmid can be used for downstream applications such as enzymatic digestion, transformation, transfection and molecular cloning etc. The beads can be an effective and inexpensive reagent for bacterial RNA depletion for routine plasmid purification.

Features

  • Effective depletion of bacterial RNA by RNase
  • High recovery rate of plasmid DNA by magnetic beads
  • Removal of unwanted components and impurities
  • Simple and fast beads-based protocol

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코아사이언스 coresciences BioDynami Korea distributor 한국 대리점 NGS Library Preparation Targeted Sequencing DNA purification magnetic beads Nucleic acid extrasction DNA RNA Quantification DNA Fragmentation DNA ladder 핵산 분리 정제 추출 시퀀싱 차세대 염기서열 분석 키트 시약 마그네틱 비드