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[마이크로RNA 분리, 정제 키트] miRNA Purification Kit (tRNA Depletion) [40057S, 40057L]_BioDynami - 코아사이언스

코피디 2023. 11. 24. 11:30

miRNA Purification Kit (tRNA Depletion)

Cat.# 40057S / Size: 24 runs

Cat.# 40057L / Size: 96 runs

Protocol download

MSDS download

 

The miRNA Purification Kit (tRNA Depletion) is developed for microRNA (miRNA) purification by removal of tRNA, 5S RNA, and other larger RNA contaminations.

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microRNAs are typically around 22 nucleotides long and can be used in various downstream applications relating to gene expression regulation, miRNA profiling, and functional analysis. However, miRNA isolated from commercial microRNA extraction kits usually contain contaminations of tRNA, 5S RNA, and other larger RNA molecules. The contamination may result in decreased sensitivity or inconsistent data for applications such as PCR, RT-PCR, next-generation sequencing (NGS), microarray, etc.

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We have developed a Solid Phase Reversible Immobilization (SPRI) beads-based technology to solve the problem. SPRI beads are well used for nucleic acid purification. The paramagnetic beads coated with carboxyl groups can reversibly bind to nucleic acid that are 100 base pairs or longer. However, with traditional SPRI beads, nucleic acids shorter than 100 bp are poorly recovered.

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Our proprietary magnetic bead technology not only overcomes the short nucleic acid recovery hurdle, but also enables effective separation of miRNA from large RNA molecules. With this technology, the kit selectively purifies miRNA and removes tRNA, 5S RNA, and other larger RNA molecules. The magnetic beads can also remove impurities and unwanted components such as salts, proteins, dNTPs, and detergents.

 

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Features

  • Purification of miRNA by removing tRNA, 5S RNA, and larger RNA contaminations
  • Removal of unwanted components and other impurities

Examples of miRNA purification

Kit efficiency: miRNA recovery rate and tRNA depletion rate

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences BioDynami Korea distributor 한국 대리점 NGS Library Preparation Targeted Sequencing DNA purification magnetic beads Nucleic acid extrasction DNA RNA Quantification DNA Fragmentation DNA ladder 핵산 분리 정제 추출 시퀀싱 차세대 염기서열 분석 키트 시약 마그네틱 비드

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