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[세포유리 DNA 분리, 정제 키트] Magnetic Beads (tRNA Purification) [40054S, 40054M, 40054L]_BioDynami - 코아사이언

코피디 2023. 11. 24. 11:06

Magnetic Beads (tRNA Purification)

Cat.# 40054S / Size: 5 mL

Cat.# 40054M / Size: 20 mL

Cat.# 40054L / Size: 100 mL

Protocol download

MSDS download

 

Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.

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The Magnetic Beads (tRNA Purification) solves the tRNA and short DNA/RNA purification problem. The beads with our proprietary technology purify tRNA effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The magnetic bead reagents can be used for oligo (>70 nt) applications.

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Workflow without large RNA/DNA contamination

 In the case of samples contaminated with RNA/DNA such as rRNA and DNA, our magnetic beads can effectively remove RNA/DNA that are 180 nt and larger. Purified tRNA are ideal for applications requiring high quality, as the fragments are free of impurities and contaminants.

Workflow with large RNA/DNA contamination

 

​Comparison of tRNA and 70 nt oligos recovery. BioDynami beads (tRNA purification) successfully recovered DNA fragments both yeast tRNA and 70 nt oligos.

Recovery of tRNA and 70 nt oligos with BioDynami magnetic Beads (tRNA Purification). Yeast tRNA and 70 nt oligos were used as input. Input and recovered oligos were quantified with ssDNA Quantification kit (BioDynami Cat. # 40043) and RNA Quantification kit (BioDynami Cat. # 40044).

Depletion of larger RNAs. Left panel: depletion of 28S rRNA and 18S rRNA. Right panel: depletion of RNA of 180 nt and larger.

 

Features

  • Purification of tRNA and oligos (>70 nt)
  • - tRNA
  • - RNA fragments 70 nt or longer
  • - DNA/RNA hybrid fragments 70 nt or longer
  • - Oligo and chimeric oligo 70 nt or longer
  • - dsDNA fragments 70 bp or longer
  • - ssDNA fragments 70 nt or longer
  • Removal of larger RNA/DNA contamination:
  • - 18S rRNA
  • - 28S rRNA
  • - RNA/DNA> 180 nt
  • - Removal of unwanted components and other impurities

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Related Products

 

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences BioDynami Korea distributor 한국 대리점 NGS Library Preparation Targeted Sequencing DNA purification magnetic beads Nucleic acid extrasction DNA RNA Quantification DNA Fragmentation DNA ladder 핵산 분리 정제 추출 시퀀싱 차세대 염기서열 분석 키트 시약 마그네틱 비드

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