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[짧은 올리고 단편 분리, 정제 마그네틱 비드] Magnetic Beads (Short Oligo Purification) [40058S, 40058L]_BioDynami - 코아사이언스

코피디 2023. 11. 24. 10:38

 

Magnetic Beads (Short Oligo Purification)

Cat.# 40058S / Size: 24 runs

Cat.# 40058L / Size: 96 runs

Protocol download

MSDS download

MSDS download

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Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.

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Oligo purification can also be performed using spin column-based technology. The oligo size limitation for recovery is around 20 nt, as oligos under 20 nt have a very low recovery rate.

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We have developed Magnetic Beads (Short Oligo Purification) Kit for short oligo purification. Our proprietary bead technology enables the recovery of oligos as short as 6 nt. 80% of the 6 nt oligos and 90% of the > 8 nt oligos can be recovered. The reagent also effectively removes impurities and unwanted components such as salts, proteins, dNTPs, detergents, and other contaminants. The magnetic bead reagents are RNase free, and can be used for both DNA and RNA applications.

Recovery rates of of short oligos. Oligos with different sizes were used as input. BioDynami Short Oligo Quantification Kit (Cat. # 40046) was used to quantify the recovery rates with modifications.

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Features

  • Effective purification of short oligos
  • 6 nt oligos: 80% recovery rate
  • >8 nt oligos: >90% recovery rate
  • Removal of impurities and unwanted reaction components

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* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences BioDynami Korea distributor 한국 대리점 NGS Library Preparation Targeted Sequencing DNA purification magnetic beads Nucleic acid extrasction DNA RNA Quantification DNA Fragmentation DNA ladder 핵산 분리 정제 추출 시퀀싱 차세대 염기서열 분석 키트 시약 마그네틱 비드

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