티스토리 뷰

Magnetic Beads (microRNA & Oligo Purification)

Cat.# 40052S / Size: 5 runs

Cat.# 40052M / Size: 200 runs

Protocol download (kit before Jul. 2023)

Protocol download (kit after Aug. 2023)

MSDS download

 

Solid Phase Reversible Immobilization magnetic beads are often used for DNA purification because they are simple, fast, and effective. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to nucleic acid. However, magnetic beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (microRNA & Oligo Purification) to solve the problem.

With our proprietary beads technology, the Magnetic Beads (microRNA & Oligo Purification) overcomes the hurdle of the short DNA/RNA recovery problem. The beads purify short DNA/RNA purification effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The magnetic bead reagents are RNase free, and can be used for both DNA and RNA applications.

Our magnetic beads are optimized to selectively purify microRNA and DNA/RNA fragments that are as short as 20 bases, such as microRNA, tRNA, dsDNA fragments 20 bp or longer, ssDNA fragments 20 nt or longer, RNA fragments 20 nt or longer, DNA/RNA hybrid fragments 20 bp or longer, oligo and chimeric oligo 20 nt or longer. Purified short DNA and RNA fragments are ideal for applications requiring high quality fragments, as the fragments are free of impurities and contaminants.

Comparison of short DNA fragments recovery. BioDynami 20 bp DNA ladder (Cat. # 10002) was used as DNA input. Ampure XP beads, BioDynami beads (DNA & RNA purification) and BioDynami beads (microRNA & Oligo purification) were tested. Only BioDynami beads (microRNA & Oligo purification) successfully recovered DNA fragments between 20-100 bp.

Recovery of DNA and RNA oligos with BioDynami magnetic Beads (microRNA & Oligo Purification). 20 nt of DNA oligos and RNA oligos were used as input. Input and recovered oligos were quantified with BioDynami ssDNA Quantification kit (Cat. # 40043) and BioDynami RNA Quantification kit (Cat. # 40044).

Features

  • Effective purification of short DNA and RNA samples
  • microRNA
  • tRNA
  • dsDNA fragments 20 bp or longer
  • ssDNA fragments 20 nt or longer
  • RNA fragments 20 nt or longer
  • DNA/RNA hybrid fragments 20 bp or longer
  • Oligo and chimeric oligo 20 nt or longer
  • Removal of impurities and unwanted reaction components
  • Compatibility: Works with both manual and automated procedures

Related Products

* 본 상품은 오직 연구용으로만 사용 가능합니다. 인체 및 제품화에 사용하실 수 없습니다.

코아사이언스 coresciences BioDynami Korea distributor 한국 대리점 NGS Library Preparation Targeted Sequencing DNA purification magnetic beads Nucleic acid extrasction DNA RNA Quantification DNA Fragmentation DNA ladder 핵산 분리 정제 추출 시퀀싱 차세대 염기서열 분석 키트 시약 마그네틱 비드