ClickTech EdU Cell Proliferation Kit 488 for FC [BCK-EdU488FC50, BCK-EdU488FC100]_Baseclick - 코아사이언스

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ClickTech EdU Cell Proliferation Kit 488 for FC

EdU Cell Proliferation Kit for Flow Cytometry

Size	Cat.#
1 Kit / 50 assays	BCK-EdU488FC50
1 Kit / 100 assays	BCK-EdU488FC100

Information

All EdU based proliferation kits on the market are patented by our company since 2007. Using EdU shows several advantages over other cell proliferation assays (BrdU or 3H-thymidine) making it a superior evaluation tool for monitoring genotoxicity, evaluation of anticancer drugs or cell cycle analysis. Measured are only the cells in vitro and/or in vivo that are actively dividing. Healthy cells that are not dividing are not detected and can thus give no false positives. The assay can be used for single cell proliferation analysis as well as cell division in animals. After incorporation of EdU, cell proliferation can be detected in different organs. The amount of EdU used in the assays shows no toxicity in cells as well as in animals thus does not influence the results of experiments.

The kits are designed for flow cytometry analysis. They contain special reagents and a dedicated protocol for read-outs using flow cytometry on cells in suspension.

The kits are available for the following wavelenghts: 488 nm; 555 nm; 594 nm; 647 nm

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LITERATURE

Cytolethal Distending Toxin Promotes Replicative Stress Leading to Genetic Instability Transmitted to Daughter Cells, W. Tremblay et al., 2021, Frontiers in Cell and Developmental Biology, Vol. 9, p. 656795.

https://doi.org/10.3389/fcell.2021.656795

Innovative DNA-Targeted Metallo-prodrug Strategy Combining Histone Deacetylase Inhibition with Oxidative Stress, T. McGivern et al., 2018, Mol. Pharmaceutics, Vol.15, p. 5058-5071.

https://doi.org/10.1021/acs.molpharmaceut.8b00652

YB-1 Expression and Phosphorylation Regulate Tumorigenicity and Invasiveness in Melanoma by Influencing EMT, C. Kosnopfel et al., 2018, Mol Cancer Res., Vol. 16, p. 1149-1160.

https://doi.org/10.1158/1541-7786.MCR-17-0528

Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response, B. J. Pons et al., 2021, Cellular and Molecular Life Sciences, Vol. 78, p. 6319–6335.

https://doi.org/10.1007/s00018-021-03902-x

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Chemical Properties

• Shelf Life: 12 months unopened after receipt
• Storage Conditions: 2-8 °C
• Physical State: kit system made of different components
• CAS Number: n.a.
• Excitation (max): 496 nm
• Emission (max): 516 nm
• Ɛ (max): 83.000 cm-1M-1
• Preparation/Handling: please see user manual of the kit

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Documents

• » User Manual (PDF)
• » Safety Data Sheet (PDF)

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For research use only!​

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