ClickTech EdU Cell Proliferation Kit 488 (100 Assays) for IM [BCK-EdU488IM100]_Baseclick - 코아사이언스

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ClickTech EdU Cell Proliferation Kit 488 (100 Assays) for IM

EdU Cell Proliferation Kit for Imaging

Size	Cat.#
1 Kit	BCK-EdU488IM100

Information

All EdU based proliferation kits on the market are patented by our company since 2007. Using EdU shows several advantages over other cell proliferation assays (BrdU or 3H-thymidine) making it a superior evaluation tool for monitoring genotoxicity, evaluation of anticancer drugs or cell cycle analysis. Measured are only the cells in vitro and/or in vivo that are actively dividing. Healthy cells that are not dividing are not detected and can thus give no false positives. The assay can be used for single cell proliferation analysis as well as cell division in animals. After incorporation of EdU, cell proliferation can be detected in different organs. The amount of EdU used in the assays shows no toxicity in cells as well as in animals thus does not influence the results of experiments.

The kits are designed for fluorescence microscopy. They contain special reagents and a dedicated protocol for read-outs using fluorescence microscopy on either cells in suspension or attached cells.

The kits are available for the following wavelenghts: 488 nm; 555 nm; 594 nm; 647 nm

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LITERATURE

Anticancer drug and ionizing radiation-induced DNA damage differently influences transcription activity and DDR-related stress responses of an endothelial monolayer, V. Ziegler et al., 2020, Molecular Cell Research, Vol. 1867, p. 118678.

https://doi.org/10.1016/j.bbamcr.2020.118678

Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ, N. Raddaoui et al. 2017, ChemBioChem, Vol. 18, p. 1716-1720.

https://doi.org/10.1002/cbic.201700209

High-copy sequences reveal distinct evolution of the rye B chromosome, S. Klemme et al., 2013, New Phytologist, Vol. 199, p. 550-558.

https://doi.org/10.1111/nph.12289

Cytolethal Distending Toxin Promotes Replicative Stress Leading to Genetic Instability Transmitted to Daughter Cells, W. Tremblay et al., 2021, Frontiers in Cell and Developmental Biology, Vol. 9, 656795.

https://doi.org/10.3389/fcell.2021.656795

Brassica napus Roots Use Different Strategies to Respond to Warm Temperatures, M. Boter et al., 2023, Int. J. Mol. Sci., Vol. 24(2), 1143.

https://doi.org/10.3390/ijms24021143

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Chemical Properties

• Shelf Life: 12 months unopened after receipt
• Storage Conditions: 2-8 °C
• Physical State: kit system made of different components
• CAS Number: n.a.
• Excitation (max): 496 nm
• Emission (max): 516 nm
• Ɛ (max): 83.000 cm-1M-1
• Preparation/Handling: please see user manual of the kit

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Documents

• User Manual (PDF)
• Safety Data Sheet (PDF)

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For research use only!

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