ClickTech EdU Cell Proliferation Kit 647 (50 Assays) for FC IV [BCK-EdU647FC50+IV]_Baseclick - 코아사이언스

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ClickTech EdU Cell Proliferation Kit 647 (50 Assays) for FC IV

In vivo EdU Cell Proliferation Kit for Flow Cytometry

Size	Cat.#
1 Kit size S (+50 mg EdU)	BCK-EdU647FC50+IV-S
1 Kit size M (+500 mg EdU)	BCK-EdU647FC50+IV-M
1 Kit size L (+1000 mg EdU)	BCK-EdU647FC50+IV-L

Information

EdU in vivo cell proliferation Kit provides a superior alternative to BrdU and [3H]-thymidine assays for detection of cell proliferation in animals.

In principal, EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog, is administered to animals. EdU is incorporated into DNA during DNA synthesis and has a clickable functionality.

Detection of cell division in tissues can be performed using microscopy, flow cytometry or in high throughput screening methods. The in vivo kits are thus designed in a sense that we provide additional EdU for the administering step. The three variants of baseclick EdU in vivo kits are:

1. Imaging kit (BCK-EdU-IM100+IV)

2. Flow cytometry kit (BCK-EdU-FC50+IV)

3. High throughput screening – HTS kit (BCK-EdU-HTS2+IV)

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LITERATURE

An efficient protocol for in vivo labeling of proliferating epithelial cells, C. Michel et al., 2018, J. Immunol. Methods, Vol. 457, p. 82-86.

https://doi.org/10.1016/j.jim.2018.03.015

Cisplatin-induced DNA double-strand breaks promote meiotic chromosome synapsis in PRDM9-controlled mouse hybrid sterility, L. Wang et al., 2018, eLife, Vol. 7, p. e42511.

https://doi.org/10.7554/eLife.42511

FXR Regulates Intestinal Cancer Stem Cell Proliferation, T. Fu et al., 2019, Cell, Vol. 176, p. 1098-1112.

https://doi.org/10.1016/j.cell.2019.01.036

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Chemical Properties

• Shelf Life: 12 months unopened after receipt
• Storage Conditions: 2-8 °C
• Physical State: kit system made of different components
• CAS Number: n.a.
• Excitation (max): 643 nm
• Emission (max): 662 nm
• Ɛ (max): 250.000 cm-1M-1
• Preparation/Handling: please see user manual of the kit

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Documents

• » User Manual Part 1 (PDF)
• » User Manual Part 2 (PDF)
• » Safety Data Sheet (PDF)

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For research use only!

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