[Ready to use laminin-511 fragment/Easy iMatrix-511 series] Easy iMatrix-511 [892018] & Easy iMatrix-511 silk [892024]_Funakoshi - 코아사이언스

No more laborious calculation for dilution of coating matrix!

Easy iMatrix-511 & Easy iMatrix-511 silk

Ready to use laminin-511 fragment

 

iMatrix-511, is the recombinant human Laminin-511 E8 fragment, produced by CHO-S cells or Transgenic silkworm cocoon. They can reduce the risk of the xeno-components contamination in the culture.

 

Compared to regular iMatrix-511, Easy iMatrix-511and Easy iMatrix-511 silk are ready to use format then do not require dilution step and laborious calculation of concentration.

 

Background of Laminin and Laminin fragment 

Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals,

and play an important role for cell adhesion and proliferation.Laminin is composed of three parts;

alpha, beta, and gamma - chain and 15 types of laminin is already known.

Among them, Laminin composed by Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-511".

 

Laminin-511 is recognized by alpha6beta1 integrin. Cell adhision, extension and proliferation is caused by many

cell signalings through intergrin.Laminin-511 E8 is the fragment of Laminin-511 obtained by enzyme digestion.

 

Although this is fragment, it has binding ability to alpha6beta1 integrin.

 

Features 

・ Ready to use
・Works in various cells - especially good for iPS / ES cells
・ Reduce the risk of contamination by xeno-components in your culture system.
・ High cell viability and proliferation rate.

 

Note: This product contains recombinant human serum albumin for stabilization and improvement of activity.

 

Advantage of using Laminin-511E8 fragment in ES/iPS cell culture 

With conventional culture methods, cells die if ES/iPS cells are dispersed into single cells.
Therefore, maintaining cell colony is necessary to a certain extent when passaging.

Using Laminin-511 E8 fragment as cell culture substrate enables to maintain and culture even a single ES/iPS.
-  The period of time required for learning cell culture methods is reduced.

-  The extension efficiency of cell culture is drastically enhanced.

 

Procedure 

In the case of 6 well culture plate ... (9.6 cm^2/well)

(1) Add 1.5 mL / well of Easy iMatrix-511 to the plate.

(2) Incubate the plate at 37℃ for a hour at RT or 4℃ for overnight.

(3) After (2), discard solution and start culture shortly. (DO NOT DRY!)

 

Caution :For iPS cells, recommended cell density is 2 - 3 x 10^3 cells/cm^2.

However, culture condition must be optimized.

 

Click here for the extended culture protocol for hES/hiPS cells using iMatrix-511. (External site.)

Click here for the video for the passage procedure for hES/hiPS cells using iMatrix-511. (External site.)

 

 

See the product information of regular iMatrix-511 from here.

Ordering Information 

Product Name	Size	Cat.#	Storage
Easy iMatrix-511	100 mL	892018	4℃
Easy iMatrix-511 silk	100 mL	892024	4℃

 

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