[Food & Water Safety][대장균 eae 유전자 검출 qPCR 키트] Escherichia coli Typing eae (EHEC or EPEC) qPCR Kit [MD06171]_NZYTech - 코아사이언스

 

Escherichia coli Typing eae (EHEC or EPEC) qPCR Kit

Cat.# MD06171 / Size: 100 reactions

Manual / MSDS

 

Escherichia coli Typing eae (EHEC or EPEC) qPCR Kit is designed for the in vitro detection of E. coli eae (EHEC or EPEC) genomes.

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Escherichia coli is a Gram-negative, facultative anaerobic, rod-shaped bacterium that is commonly found in the lower intestine of humans and animals. Most E. coli strains are harmless, however some serotypes, such as Enterohaemorrhagic (EHEC) and Enteropathogenic Escherichia coli (EPEC), can cause serious food poisoning in their hosts.

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Escherichia coli Typing eae (EHEC or EPEC) qPCR Kit is designed for the in vitro detection of E. coli eae (EHEC or EPEC) genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to E. coli eae (EHEC or EPEC). Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of E. coli eae (EHEC or EPEC) genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase(see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Escherichia coli Typing eae (EHEC or EPEC)
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Duplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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