[Food & Water Safety][대장균 stx1/stx2 유전자 검출 qPCR 키트] Escherichia coli Typing stx1 & stx2 (STEC) qPCR Kit [MD06201]_NZYTech - 코아사이언스

 

Escherichia coli Typing stx1 & stx2 (STEC) qPCR Kit

Cat.# MD06201 / Size: 100 reactions

Manual / MSDS

Escherichia coli Typing stx1 & stx2 (STEC) qPCR Kit is designed for the in vitro detection of E. coli stx1 and stx2 genes.

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Shiga toxin producing Escherichia coli (STEC) are Gram-negative and facultative anaerobic bacteria. They are foodborne pathogens of public health importance that cause diarrhea, hemorrhagic colitis and hemolytic uremic syndrome in humans. The main virulence factors of STEC are Shiga toxins (Stx1 and Stx2) and additionally they can also synthesize the adhesin intimin (encoded by eae), an enterohemolysin (EhxA), and an autoagglutinating protein (Saa), among other virulence factors. Cattle and sheep constitute natural reservoirs of these STEC strains and transmission to humans can happen through direct contact with infected animals and consumption of contaminated food and water.

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Escherichia coli Typing stx1 & stx2 (STEC) qPCR Kit is designed for the in vitro detection of STEC genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to STEC. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of STEC genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

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If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

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Components:

- Lyo NZYSupreme qPCR master mix (2x)

- qPCR master mix reconstitution buffer

- Lyo PPMix (10x)

- NTC

- Positive Control

- DNA Internal Extraction Control (IEC)

 

Shipping Conditions	Room Temperature
Storage Conditions	-85 °C to -15 °C
Format	Lyophilized
Product Category	Predesigned qPCR assays
Compatibility	All standard Real-time thermal cyclers
Target Species	Escherichia coli Typing stx1 & stx2 (STEC)
Sample Material	DNA
Protocol time	Approx. 60 min (from extracted NA to result)
Number of targets	Triplex
Internal Control	No
Features	Free automatic data analyzer (Fastfinder)
Detection Method	Probe Based

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